ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias
Specific binding of radiolabeled inhibitor was employed to localize the Na-pump sites (Na,K-ATPase) in rectal gland epithelium, a NaCl-secreting osmoregulatory tissue which is particularly rich in pump sites. Slices of gland tissue from spiny dogfish were incubated in suitable [3H]ouabain-containing...
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.5718 |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.280.5718 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.280.5718 2023-05-15T18:51:07+02:00 ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias Jill Eveloff Karl J Karnaky Patricio Silva Franklin H Epstein William B Kinter The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.5718 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.5718 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/b9/c2/J_Cell_Biol_1979_Oct_1_83(1)_16-32.tar.gz text ftciteseerx 2016-01-07T21:03:41Z Specific binding of radiolabeled inhibitor was employed to localize the Na-pump sites (Na,K-ATPase) in rectal gland epithelium, a NaCl-secreting osmoregulatory tissue which is particularly rich in pump sites. Slices of gland tissue from spiny dogfish were incubated in suitable [3H]ouabain-containing media and then prepared for Na,K-ATPase assay, measurement of radiolabel binding, or quantitative freeze-dry autoradiography at the light microscope level. Gross freezing or drying artifacts were excluded by comparison with additional aldehyde-fixed slices. Characterization experiments demonstrated high-affinity binding which correlated with Na,K-ATPase inhibition and half-saturated at-5 p,M [3H]ouabain. At this concentration, the normal half-loading time was-1 h and low-affinity binding to nonspecific sites was negligible. Autoradiographs from both 1- and 4-h incubated slices showed ^-85 % of the bound [ 3H]ouabain to be localized within a 1-Lm wide boundary region where the highly infolded basal-lateral cell membranes are closest Text spiny dogfish Squalus acanthias Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
Specific binding of radiolabeled inhibitor was employed to localize the Na-pump sites (Na,K-ATPase) in rectal gland epithelium, a NaCl-secreting osmoregulatory tissue which is particularly rich in pump sites. Slices of gland tissue from spiny dogfish were incubated in suitable [3H]ouabain-containing media and then prepared for Na,K-ATPase assay, measurement of radiolabel binding, or quantitative freeze-dry autoradiography at the light microscope level. Gross freezing or drying artifacts were excluded by comparison with additional aldehyde-fixed slices. Characterization experiments demonstrated high-affinity binding which correlated with Na,K-ATPase inhibition and half-saturated at-5 p,M [3H]ouabain. At this concentration, the normal half-loading time was-1 h and low-affinity binding to nonspecific sites was negligible. Autoradiographs from both 1- and 4-h incubated slices showed ^-85 % of the bound [ 3H]ouabain to be localized within a 1-Lm wide boundary region where the highly infolded basal-lateral cell membranes are closest |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Jill Eveloff Karl J Karnaky Patricio Silva Franklin H Epstein William B Kinter |
spellingShingle |
Jill Eveloff Karl J Karnaky Patricio Silva Franklin H Epstein William B Kinter ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
author_facet |
Jill Eveloff Karl J Karnaky Patricio Silva Franklin H Epstein William B Kinter |
author_sort |
Jill Eveloff |
title |
ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
title_short |
ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
title_full |
ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
title_fullStr |
ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
title_full_unstemmed |
ELASMOBRANCH RECTAL GLAND CELL Autoradiographic Localization of [3 HjOuabain- Sensitive Na, K-ATPase in Rectal Gland of Dogfish, Squalus Acanthias |
title_sort |
elasmobranch rectal gland cell autoradiographic localization of [3 hjouabain- sensitive na, k-atpase in rectal gland of dogfish, squalus acanthias |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.5718 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/b9/c2/J_Cell_Biol_1979_Oct_1_83(1)_16-32.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.5718 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766244909800488960 |