ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins
The adhesion of artificially generated lipid membrane vesicles to Chinese hamster V79 fibroblasts in suspension was used as a model system for studying membrane interactions. Below their gel-liquid crystalline phase transition temperature, vesicles comprised of dipalmitoyl lecithin (DPL) or dimyrist...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.280.4168 2023-05-15T16:01:24+02:00 ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins Richard E. Pagano Masatoshi Takeichi The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4168 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4168 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/74/08/J_Cell_Biol_1977_Aug_1_74(2)_531-546.tar.gz text ftciteseerx 2016-01-07T21:03:08Z The adhesion of artificially generated lipid membrane vesicles to Chinese hamster V79 fibroblasts in suspension was used as a model system for studying membrane interactions. Below their gel-liquid crystalline phase transition temperature, vesicles comprised of dipalmitoyl lecithin (DPL) or dimyristoyl lecithin (DML) adsorbed to the surfaces of EDTA-dissociated cells. These adherent vesicles could not be removed by repeated washings of the treated cells but could be released into the medium by treatment with trypsin. EM autoradiographic studies of cells treated with [3H]DML or [3H]DPL vesicles showed that most of the radioactive lipids were confined to the cell periphery. Scanning electron microscopy and fluorescence microscopy further confirmed the presence of adherent vesicles at the cell surface. Adhesion of DML or DPL vesicles to EDTA-dissociated cells modified the lactoperoxidase-catalyzed iodination pattern of the cell surface proteins; the inhibition of labeling of two proteins with an-60,000-dalton mol wt was particularly Text DML Unknown |
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English |
description |
The adhesion of artificially generated lipid membrane vesicles to Chinese hamster V79 fibroblasts in suspension was used as a model system for studying membrane interactions. Below their gel-liquid crystalline phase transition temperature, vesicles comprised of dipalmitoyl lecithin (DPL) or dimyristoyl lecithin (DML) adsorbed to the surfaces of EDTA-dissociated cells. These adherent vesicles could not be removed by repeated washings of the treated cells but could be released into the medium by treatment with trypsin. EM autoradiographic studies of cells treated with [3H]DML or [3H]DPL vesicles showed that most of the radioactive lipids were confined to the cell periphery. Scanning electron microscopy and fluorescence microscopy further confirmed the presence of adherent vesicles at the cell surface. Adhesion of DML or DPL vesicles to EDTA-dissociated cells modified the lactoperoxidase-catalyzed iodination pattern of the cell surface proteins; the inhibition of labeling of two proteins with an-60,000-dalton mol wt was particularly |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Richard E. Pagano Masatoshi Takeichi |
spellingShingle |
Richard E. Pagano Masatoshi Takeichi ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
author_facet |
Richard E. Pagano Masatoshi Takeichi |
author_sort |
Richard E. Pagano |
title |
ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
title_short |
ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
title_full |
ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
title_fullStr |
ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
title_full_unstemmed |
ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins |
title_sort |
adhesion of phospholipid vesicles to chinese hamster fibroblasts role of cell surface proteins |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4168 |
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DML |
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DML |
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ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/74/08/J_Cell_Biol_1977_Aug_1_74(2)_531-546.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4168 |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766397280668090368 |