ADHESION OF PHOSPHOLIPID VESICLES TO CHINESE HAMSTER FIBROBLASTS Role of Cell Surface Proteins

• Main Authors: Richard E. Pagano, Masatoshi Takeichi
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: DML
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.280.4168

The adhesion of artificially generated lipid membrane vesicles to Chinese hamster V79 fibroblasts in suspension was used as a model system for studying membrane interactions. Below their gel-liquid crystalline phase transition temperature, vesicles comprised of dipalmitoyl lecithin (DPL) or dimyristoyl lecithin (DML) adsorbed to the surfaces of EDTA-dissociated cells. These adherent vesicles could not be removed by repeated washings of the treated cells but could be released into the medium by treatment with trypsin. EM autoradiographic studies of cells treated with [3H]DML or [3H]DPL vesicles showed that most of the radioactive lipids were confined to the cell periphery. Scanning electron microscopy and fluorescence microscopy further confirmed the presence of adherent vesicles at the cell surface. Adhesion of DML or DPL vesicles to EDTA-dissociated cells modified the lactoperoxidase-catalyzed iodination pattern of the cell surface proteins; the inhibition of labeling of two proteins with an-60,000-dalton mol wt was particularly