An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus)
with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the par...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.279.1388 2023-05-15T13:19:45+02:00 An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) J. Åkerstedt The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.279.1388 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.279.1388 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/6e/90/Acta_Vet_Scand_2002_Dec_31_43(4)_211-220.tar.gz text ftciteseerx 2016-01-07T20:57:39Z with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ=0.99), and the ELISA and IFAT (κ=0.958). There was no significant statistical difference between the tests (p>0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. microsporidia; encephalitozoonosis; ELISA; carbon immunoassay; indirect immunofluorescence. Text Alopex lagopus Unknown |
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ftciteseerx |
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English |
description |
with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus). The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ=0.99), and the ELISA and IFAT (κ=0.958). There was no significant statistical difference between the tests (p>0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. microsporidia; encephalitozoonosis; ELISA; carbon immunoassay; indirect immunofluorescence. |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
J. Åkerstedt |
spellingShingle |
J. Åkerstedt An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
author_facet |
J. Åkerstedt |
author_sort |
J. Åkerstedt |
title |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_short |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_fullStr |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_full_unstemmed |
An Indirect ELISA for Detection of Encephalitozoon cuniculi Infection in Farmed Blue Foxes (Alopex lagopus) |
title_sort |
indirect elisa for detection of encephalitozoon cuniculi infection in farmed blue foxes (alopex lagopus) |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.279.1388 |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/6e/90/Acta_Vet_Scand_2002_Dec_31_43(4)_211-220.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.279.1388 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766349382958972928 |