The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin
T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110-121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) VO usage in DBA/2 mice. All clones previously tested used the same V(3 8.2 gene segme...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.274.162 2023-05-15T18:26:48+02:00 The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin Giovina Ruberti Amitabh Gaur C Garrison Fathman Ra M Livingstone The Pennsylvania State University CiteSeerX Archives application/zip http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.274.162 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.274.162 Metadata may be used without restrictions as long as the oai identifier remains attached to it. ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/d6/7f/J_Exp_Med_1991_Jul_1_174(1)_83-92.tar.gz text ftciteseerx 2016-01-07T20:45:13Z T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110-121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) VO usage in DBA/2 mice. All clones previously tested used the same V(3 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpWMb protein or peptide 110-121. Only the V#8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V0 genes, lacked V08+ peripheral T cells. DBA/2 mice depleted of V08+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2Vf' mice (homozygous for a deletion ofmultiple VO gene segments including the V#8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V0°mice had a very restricted TCR Vii repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2VO, and F23.1-treated DBA/2 mice are discussed. Text Sperm whale Unknown |
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English |
description |
T cell clones recognizing the sperm whale myoglobin (SpWMb) epitope 110-121 in association with H-2d major histocompatibility complex class II molecules display a very limited heterogeneity of T cell receptor (TCR) VO usage in DBA/2 mice. All clones previously tested used the same V(3 8.2 gene segment and very restricted junctional regions. To investigate the significance of this observation in vivo, we immunized DBA/2 mice with the intact SpWMb protein or peptide 110-121. Only the V#8+ T cells showed any significant response to the 110-121 epitope. The response to peptide 110-121 was then analyzed in mice which, either as a consequence of antibody depletion or through genetic deletion of TCR V0 genes, lacked V08+ peripheral T cells. DBA/2 mice depleted of V08+ T cells by antibody treatment responded poorly to the 110-121 peptide, and only at high antigen concentrations. In contrast, DBA/2Vf' mice (homozygous for a deletion ofmultiple VO gene segments including the V#8 family) made a response at least as great as that made by DBA/2 mice, even though the DBA/2V0°mice had a very restricted TCR Vii repertoire compared with DBA/2 mice. Mechanisms which might determine differences in the 110-121 specific response of DBA/2, DBA/2VO, and F23.1-treated DBA/2 mice are discussed. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Giovina Ruberti Amitabh Gaur C Garrison Fathman Ra M Livingstone |
spellingShingle |
Giovina Ruberti Amitabh Gaur C Garrison Fathman Ra M Livingstone The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
author_facet |
Giovina Ruberti Amitabh Gaur C Garrison Fathman Ra M Livingstone |
author_sort |
Giovina Ruberti |
title |
The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
title_short |
The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
title_full |
The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
title_fullStr |
The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
title_full_unstemmed |
The T Cell Receptor Repertoire Influences VO Element Usage in Response to Myoglobin |
title_sort |
t cell receptor repertoire influences vo element usage in response to myoglobin |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.274.162 |
genre |
Sperm whale |
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Sperm whale |
op_source |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/d6/7f/J_Exp_Med_1991_Jul_1_174(1)_83-92.tar.gz |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.274.162 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766208772563271680 |