Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species

• Main Authors: Mona Stancheva, Diana A. Dobreva, Bistra Galunska
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: retinol; cholecalciferol; alpha-tocopherol; turbot; garfish; HPLC; Turbot
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.232.7748; http://www.univ-ovidius.ro/anale-chimie/chemistry/2012/full/4_dobreva.pdf

Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes- Garfish (Belone belone) and Turbot (Psetta maxima). All-trans-retinol (vitamin A), cholecalciferol (vitamin D3) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at λmax = 325 nm and λmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at λex=288 nm and λem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values.