Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species
Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes- Garfish (Belone belone) and Turbot (Psetta maxima). All-trans-retinol (vitamin A), cholecalciferol (vitamin D3) and alpha-tocoph...
Main Authors: | , , |
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Format: | Text |
Language: | English |
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Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.232.7748 http://www.univ-ovidius.ro/anale-chimie/chemistry/2012/full/4_dobreva.pdf |
Summary: | Abstract The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes- Garfish (Belone belone) and Turbot (Psetta maxima). All-trans-retinol (vitamin A), cholecalciferol (vitamin D3) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at λmax = 325 nm and λmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at λex=288 nm and λem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values. |
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