Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica
ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial communi...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1090.764 2023-05-15T13:43:21+02:00 Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica Reut Sorek Abramovich Francesco Pomati Anne D Jungblut Mauro Guglielmin Brett A Neilan The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1090.764 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1090.764 Metadata may be used without restrictions as long as the oai identifier remains attached to it. https://www.aca.unsw.edu.au/sites/default/files/publications/ppp1749.pdf text ftciteseerx 2020-05-24T00:23:38Z ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal-restriction fragment length polymorphism (T-RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice-core samples ranged from 0.29 ng mL -1 in Amorphous Glacier to 88 ng mL -1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. Text Antarc* Antarctica Ice ice core permafrost Victoria Land Unknown Amorphous Glacier ENVELOPE(163.933,163.933,-74.700,-74.700) Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) Victoria Land |
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Open Polar |
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Unknown |
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ftciteseerx |
language |
English |
description |
ABSTRACT The debris cones known as Amorphous Glacier and Boulder Clay are located in an ice-free region in Northern Victoria Land, Antarctica, and differ in their isotopic composition, mechanisms of ice distribution, geological formation and age. However, to date it is not known if bacterial community profiles within ice and permafrost can be established for these environments, and then whether glaciological differences between the two areas would be reflected in the bacterial community composition. In order to gather first evidence for the bacterial communities in these glacial zones, we carried out terminal-restriction fragment length polymorphism (T-RFLP) analysis on the 16S rRNA gene using a universal bacterial amplification protocol on two permafrost cores. The DNA yields from ice-core samples ranged from 0.29 ng mL -1 in Amorphous Glacier to 88 ng mL -1 in Boulder Clay. Bray-Curtis cluster analysis suggested Boulder Clay bacterial profiles were similar to each other, but cluster separately from the Amorphous Glacier bacterial profile. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Reut Sorek Abramovich Francesco Pomati Anne D Jungblut Mauro Guglielmin Brett A Neilan |
spellingShingle |
Reut Sorek Abramovich Francesco Pomati Anne D Jungblut Mauro Guglielmin Brett A Neilan Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
author_facet |
Reut Sorek Abramovich Francesco Pomati Anne D Jungblut Mauro Guglielmin Brett A Neilan |
author_sort |
Reut Sorek Abramovich |
title |
Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_short |
Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_full |
Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_fullStr |
Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_full_unstemmed |
Short Communication T-RFLP Fingerprinting Analysis of Bacterial Communities in Debris Cones, Northern Victoria Land, Antarctica |
title_sort |
short communication t-rflp fingerprinting analysis of bacterial communities in debris cones, northern victoria land, antarctica |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1090.764 |
long_lat |
ENVELOPE(163.933,163.933,-74.700,-74.700) ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Amorphous Glacier Bray Victoria Land |
geographic_facet |
Amorphous Glacier Bray Victoria Land |
genre |
Antarc* Antarctica Ice ice core permafrost Victoria Land |
genre_facet |
Antarc* Antarctica Ice ice core permafrost Victoria Land |
op_source |
https://www.aca.unsw.edu.au/sites/default/files/publications/ppp1749.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1090.764 |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766187872214319104 |