Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes?
ABSTRACT: We have obtained deuterium NMR spin-echo spectra of crystalline ~-[ y -~H~] v a l i n e , [S-methyl-*H3]-methionine, cyanoferrimyoglobin from sperm whale (Physeter catodon), containing deuteriomethyl groups at methionine-55 and methionine-1 3 1, and [y-2H6]valine-labeled bacteriorhodopsin...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1083.822 2023-05-15T18:26:51+02:00 Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? Ion C Baianu H S Gutowsky Eric Oldfield The Pennsylvania State University CiteSeerX Archives 1984 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1083.822 http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1083.822 http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf text 1984 ftciteseerx 2020-05-03T00:29:21Z ABSTRACT: We have obtained deuterium NMR spin-echo spectra of crystalline ~-[ y -~H~] v a l i n e , [S-methyl-*H3]-methionine, cyanoferrimyoglobin from sperm whale (Physeter catodon), containing deuteriomethyl groups at methionine-55 and methionine-1 3 1, and [y-2H6]valine-labeled bacteriorhodopsin in the purple membrane of Halobacterium halobium R1. By using 90-~-&,~ (XU) and 90-rfloe (XX) pulse sequences and observing the dependence of the spin-echo amplitude upon the interpulse spacing T , we have determined that the so-called "quadrupole echoes" obtained in these typical selectively deuterated condensed-phase biological systems are in fact strongly modulated by proton-deuteron and deuteron-deuteron dipolar interactions. The two amino acids and the protein crystals behaved as typical organic solids, with no F o r over 10 years now, there has been considerable interest in the use of deuterium (*H) nuclear magnetic resonance (NMR)' as a tool for probing the static and dynamic structures of cell membranes Many groups are now using 2H NMR of selectively 2H-labeled compounds to study the static and dynamic structures of various biological systems Theory NMR spin echoes from a quadrupolar nucleus in a solid were first observed and calculated by Subsequently, however, deuterium NMR spin echoes were observed for such a 90-7-floo sequence (Boden et al., 1978), when / 3 # k X 90° and k = 0, 1,2, . the observation of spin echoes with a 9O-r-flo0 sequence in perdeuterated organic solids proves that dipolar interactions between deuterons, at least in these systems, cannot be neglected since such echoes arise only when dipolar interactions are present Text Sperm whale Unknown Boden ENVELOPE(21.683,21.683,65.809,65.809) Catodon ENVELOPE(-59.966,-59.966,-63.500,-63.500) |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
ABSTRACT: We have obtained deuterium NMR spin-echo spectra of crystalline ~-[ y -~H~] v a l i n e , [S-methyl-*H3]-methionine, cyanoferrimyoglobin from sperm whale (Physeter catodon), containing deuteriomethyl groups at methionine-55 and methionine-1 3 1, and [y-2H6]valine-labeled bacteriorhodopsin in the purple membrane of Halobacterium halobium R1. By using 90-~-&,~ (XU) and 90-rfloe (XX) pulse sequences and observing the dependence of the spin-echo amplitude upon the interpulse spacing T , we have determined that the so-called "quadrupole echoes" obtained in these typical selectively deuterated condensed-phase biological systems are in fact strongly modulated by proton-deuteron and deuteron-deuteron dipolar interactions. The two amino acids and the protein crystals behaved as typical organic solids, with no F o r over 10 years now, there has been considerable interest in the use of deuterium (*H) nuclear magnetic resonance (NMR)' as a tool for probing the static and dynamic structures of cell membranes Many groups are now using 2H NMR of selectively 2H-labeled compounds to study the static and dynamic structures of various biological systems Theory NMR spin echoes from a quadrupolar nucleus in a solid were first observed and calculated by Subsequently, however, deuterium NMR spin echoes were observed for such a 90-7-floo sequence (Boden et al., 1978), when / 3 # k X 90° and k = 0, 1,2, . the observation of spin echoes with a 9O-r-flo0 sequence in perdeuterated organic solids proves that dipolar interactions between deuterons, at least in these systems, cannot be neglected since such echoes arise only when dipolar interactions are present |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Ion C Baianu H S Gutowsky Eric Oldfield |
spellingShingle |
Ion C Baianu H S Gutowsky Eric Oldfield Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
author_facet |
Ion C Baianu H S Gutowsky Eric Oldfield |
author_sort |
Ion C Baianu |
title |
Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
title_short |
Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
title_full |
Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
title_fullStr |
Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
title_full_unstemmed |
Origin and Behavior of Deuteron Spin Echoes in Selectively Labeled Amino Acids, Myoglobin Microcrystals, and Purple Membranes? |
title_sort |
origin and behavior of deuteron spin echoes in selectively labeled amino acids, myoglobin microcrystals, and purple membranes? |
publishDate |
1984 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1083.822 http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf |
long_lat |
ENVELOPE(21.683,21.683,65.809,65.809) ENVELOPE(-59.966,-59.966,-63.500,-63.500) |
geographic |
Boden Catodon |
geographic_facet |
Boden Catodon |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1083.822 http://feh.scs.uiuc.edu/doc/papers/1201228224_79.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766208820298645504 |