PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC
Abstract Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTK...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1082.7081 2023-05-15T16:33:03+02:00 PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC Jennifer C C Neale Thomas P Kenny Ronald S Tjeerdema & M Eric Gershwin The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1082.7081 http://downloads.hindawi.com/journals/jir/2005/786243.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1082.7081 http://downloads.hindawi.com/journals/jir/2005/786243.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://downloads.hindawi.com/journals/jir/2005/786243.pdf text ftciteseerx 2020-05-03T00:27:25Z Abstract Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs) Fyn and Itk, which play a critical role in T cell activation, and IL-2, a cytokine of central importance in initiating adaptive immune responses. IL-1, the macrophage-derived pro-inflammatory cytokine of innate immunity, was also included as a measure of macrophage function. Harbor seal PBMC were exposed to the prototypic immunotoxic PAH benzo[a ]pyrene (BaP), 3,3 0 ,4,4 0 ,5,5 0 -hexachlorobiphenyl (CB-169), a model immunotoxic PCB, or DMSO (vehicle control). Exposure of Con A-stimulated harbor seal PBMC to both BaP and CB-169 produced significantly altered expression in all four targets relative to vehicle controls. The PTKs Fyn and Itk were both up-regulated following exposure to BaP and CB-169. In contrast, transcripts for IL-2 and IL-1 were decreased relative to controls by both treatments. Our findings are consistent with those of previous researchers working with human and rodent systems and support a hypothesis of contaminant-altered lymphocyte function mediated (at least in part) by disruption of T cell receptor (TCR) signaling and cytokine production. Text harbor seal Phoca vitulina Unknown Fyn ENVELOPE(20.735,20.735,69.763,69.763) |
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Abstract Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs) Fyn and Itk, which play a critical role in T cell activation, and IL-2, a cytokine of central importance in initiating adaptive immune responses. IL-1, the macrophage-derived pro-inflammatory cytokine of innate immunity, was also included as a measure of macrophage function. Harbor seal PBMC were exposed to the prototypic immunotoxic PAH benzo[a ]pyrene (BaP), 3,3 0 ,4,4 0 ,5,5 0 -hexachlorobiphenyl (CB-169), a model immunotoxic PCB, or DMSO (vehicle control). Exposure of Con A-stimulated harbor seal PBMC to both BaP and CB-169 produced significantly altered expression in all four targets relative to vehicle controls. The PTKs Fyn and Itk were both up-regulated following exposure to BaP and CB-169. In contrast, transcripts for IL-2 and IL-1 were decreased relative to controls by both treatments. Our findings are consistent with those of previous researchers working with human and rodent systems and support a hypothesis of contaminant-altered lymphocyte function mediated (at least in part) by disruption of T cell receptor (TCR) signaling and cytokine production. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Jennifer C C Neale Thomas P Kenny Ronald S Tjeerdema & M Eric Gershwin |
spellingShingle |
Jennifer C C Neale Thomas P Kenny Ronald S Tjeerdema & M Eric Gershwin PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
author_facet |
Jennifer C C Neale Thomas P Kenny Ronald S Tjeerdema & M Eric Gershwin |
author_sort |
Jennifer C C Neale |
title |
PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
title_short |
PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
title_full |
PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
title_fullStr |
PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
title_full_unstemmed |
PAH-and PCB-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (Phoca vitulina) PBMC |
title_sort |
pah-and pcb-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (phoca vitulina) pbmc |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1082.7081 http://downloads.hindawi.com/journals/jir/2005/786243.pdf |
long_lat |
ENVELOPE(20.735,20.735,69.763,69.763) |
geographic |
Fyn |
geographic_facet |
Fyn |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
http://downloads.hindawi.com/journals/jir/2005/786243.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1082.7081 http://downloads.hindawi.com/journals/jir/2005/786243.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766022767518416896 |