Molecular Detection of Past Pathogens
Abstract Detection and characterisation of DNA is the most widely used approach for the study of past pathogens. This approach can be applied to various specimens, including environmental, vector and animal reservoir specimens as well as human corpses. Experimental data indicated that host-associate...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1081.8116 2023-05-15T17:57:45+02:00 Molecular Detection of Past Pathogens Michel Drancourt Didier Raoult The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1081.8116 http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1081.8116 http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf text ftciteseerx 2020-05-03T00:25:55Z Abstract Detection and characterisation of DNA is the most widely used approach for the study of past pathogens. This approach can be applied to various specimens, including environmental, vector and animal reservoir specimens as well as human corpses. Experimental data indicated that host-associated microbial DNA can survive for 20,000 years, and bacterial DNA preserved in permafrost specimens has been dated up to 1 million years. Current protocols targeted one pathogen at a time and universal 16S rDNA-based detection of bacteria have yielded ambiguous results. There is no universal detection of ancient virus so far. Major human pathogens, e.g. Mycobacterium tuberculosis, Mycobacterium leprae, Yersinia pestis, Rickettsia prowazekii, Bartonella spp. and Spanish influenza virus have been detected in suitable human specimens. Ancient M. tuberculosis and Y. pestis organisms have been genotyped, whereas the entire RNA genome of Spanish influenza virus was reconstituted for extensive studies. Metagenomic approaches based on high throughput pyrosequencing may help further resolve forthcoming issues. Interpretation of experimental data has to be based upon strict rules due to potential contamination of specimens. Text permafrost Unknown |
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Abstract Detection and characterisation of DNA is the most widely used approach for the study of past pathogens. This approach can be applied to various specimens, including environmental, vector and animal reservoir specimens as well as human corpses. Experimental data indicated that host-associated microbial DNA can survive for 20,000 years, and bacterial DNA preserved in permafrost specimens has been dated up to 1 million years. Current protocols targeted one pathogen at a time and universal 16S rDNA-based detection of bacteria have yielded ambiguous results. There is no universal detection of ancient virus so far. Major human pathogens, e.g. Mycobacterium tuberculosis, Mycobacterium leprae, Yersinia pestis, Rickettsia prowazekii, Bartonella spp. and Spanish influenza virus have been detected in suitable human specimens. Ancient M. tuberculosis and Y. pestis organisms have been genotyped, whereas the entire RNA genome of Spanish influenza virus was reconstituted for extensive studies. Metagenomic approaches based on high throughput pyrosequencing may help further resolve forthcoming issues. Interpretation of experimental data has to be based upon strict rules due to potential contamination of specimens. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Michel Drancourt Didier Raoult |
spellingShingle |
Michel Drancourt Didier Raoult Molecular Detection of Past Pathogens |
author_facet |
Michel Drancourt Didier Raoult |
author_sort |
Michel Drancourt |
title |
Molecular Detection of Past Pathogens |
title_short |
Molecular Detection of Past Pathogens |
title_full |
Molecular Detection of Past Pathogens |
title_fullStr |
Molecular Detection of Past Pathogens |
title_full_unstemmed |
Molecular Detection of Past Pathogens |
title_sort |
molecular detection of past pathogens |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1081.8116 http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf |
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permafrost |
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permafrost |
op_source |
http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1081.8116 http://www.academia.dk/BiologiskAntropologi/Paleopatologi/PDF/Paleomicrobiology/04.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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