Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray
Abstract Sentinel fish hornyhead turbot (Pleuronichthys verticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology an...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1079.8898 2023-05-15T18:40:58+02:00 Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray M E Baker D E Vidal-Dorsch C Ribecco L J Sprague M Angert The Pennsylvania State University CiteSeerX Archives 2013 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1079.8898 http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1079.8898 http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf text 2013 ftciteseerx 2020-05-03T00:21:13Z Abstract Sentinel fish hornyhead turbot (Pleuronichthys verticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. Text Turbot Unknown |
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Abstract Sentinel fish hornyhead turbot (Pleuronichthys verticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
M E Baker D E Vidal-Dorsch C Ribecco L J Sprague M Angert |
spellingShingle |
M E Baker D E Vidal-Dorsch C Ribecco L J Sprague M Angert Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
author_facet |
M E Baker D E Vidal-Dorsch C Ribecco L J Sprague M Angert |
author_sort |
M E Baker |
title |
Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
title_short |
Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
title_full |
Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
title_fullStr |
Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
title_full_unstemmed |
Molecular Analysis of Endocrine Disruption in Hornyhead Turbot at Wastewater Outfalls in Southern California Using a Second Generation Multi-Species Microarray |
title_sort |
molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray |
publishDate |
2013 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1079.8898 http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf |
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Turbot |
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Turbot |
op_source |
http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1079.8898 http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/783_MolecularAnalysis.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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