CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci
Abstract Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8)...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1073.6014 2023-05-15T17:45:01+02:00 CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci Geena Varghese Paramel Lasse Folkersen Rona J Strawbridge Ali Ateia Elmabsout Eva Särndahl Pia Lundman Jan-Håkan Jansson Göran K Hansson Allan Sirsjö Karin Fransén The Pennsylvania State University CiteSeerX Archives 2013 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.6014 http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.6014 http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf text 2013 ftciteseerx 2020-04-26T00:26:11Z Abstract Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8) protein modulates this process by suppressing caspase 1 and the transcription factor NF-κB (nuclear factor κB). The expression of CARD8 mRNA was examined in atherosclerotic vascular tissue and the impact on MI (myocardial infarction) of a polymorphism in the CARD8 gene determined. CARD8 mRNA was analysed by microarray of human atherosclerotic tissue and compared with transplant donor arterial tissue. Microarray analysis was performed for proximal genes associated with the rs2043211 locus in plaque. The CARD8 rs2043211 polymorphism was analysed by genotyping of two Swedish MI cohorts, FIA (First Myocardial Infarction in Northern Sweden) and SCARF (Stockholm Coronary Atherosclerosis Risk Factor). The CRP (C-reactive protein) level was measured in both cohorts, but the levels of the pro-inflammatory cytokines IL-1β, IL-18, TNF (tumour necrosis factor) and MCP-1 (monocyte chemoattractant protein) were measured in sera available from the SCARF cohort. CARD8 mRNA was highly expressed in atherosclerotic plaques compared with the expression in transplant donor vessel (P < 0.00001). The minor allele was associated with lower expression of CARD8 in the plaques, suggesting that CARD8 may promote inflammation. Carriers of the minor allele of the rs2043211 polymorphism also displayed lower circulating CRP and lower levels of the pro-atherosclerotic chemokine MCP-1. However, no significant association could be detected between this polymorphism and MI in the two cohorts. Genetic alterations in the CARD8 gene therefore seem to be of limited importance for the development of MI. Text Northern Sweden Unknown |
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Abstract Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8) protein modulates this process by suppressing caspase 1 and the transcription factor NF-κB (nuclear factor κB). The expression of CARD8 mRNA was examined in atherosclerotic vascular tissue and the impact on MI (myocardial infarction) of a polymorphism in the CARD8 gene determined. CARD8 mRNA was analysed by microarray of human atherosclerotic tissue and compared with transplant donor arterial tissue. Microarray analysis was performed for proximal genes associated with the rs2043211 locus in plaque. The CARD8 rs2043211 polymorphism was analysed by genotyping of two Swedish MI cohorts, FIA (First Myocardial Infarction in Northern Sweden) and SCARF (Stockholm Coronary Atherosclerosis Risk Factor). The CRP (C-reactive protein) level was measured in both cohorts, but the levels of the pro-inflammatory cytokines IL-1β, IL-18, TNF (tumour necrosis factor) and MCP-1 (monocyte chemoattractant protein) were measured in sera available from the SCARF cohort. CARD8 mRNA was highly expressed in atherosclerotic plaques compared with the expression in transplant donor vessel (P < 0.00001). The minor allele was associated with lower expression of CARD8 in the plaques, suggesting that CARD8 may promote inflammation. Carriers of the minor allele of the rs2043211 polymorphism also displayed lower circulating CRP and lower levels of the pro-atherosclerotic chemokine MCP-1. However, no significant association could be detected between this polymorphism and MI in the two cohorts. Genetic alterations in the CARD8 gene therefore seem to be of limited importance for the development of MI. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Geena Varghese Paramel Lasse Folkersen Rona J Strawbridge Ali Ateia Elmabsout Eva Särndahl Pia Lundman Jan-Håkan Jansson Göran K Hansson Allan Sirsjö Karin Fransén |
spellingShingle |
Geena Varghese Paramel Lasse Folkersen Rona J Strawbridge Ali Ateia Elmabsout Eva Särndahl Pia Lundman Jan-Håkan Jansson Göran K Hansson Allan Sirsjö Karin Fransén CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
author_facet |
Geena Varghese Paramel Lasse Folkersen Rona J Strawbridge Ali Ateia Elmabsout Eva Särndahl Pia Lundman Jan-Håkan Jansson Göran K Hansson Allan Sirsjö Karin Fransén |
author_sort |
Geena Varghese Paramel |
title |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
title_short |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
title_full |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
title_fullStr |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
title_full_unstemmed |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. Clin. Sci |
title_sort |
card8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation. clin. sci |
publishDate |
2013 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.6014 http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.6014 http://www.clinsci.org/content/ppclinsci/125/8/401.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766147742902517760 |