Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”.
Objectives: To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods: A collection of 341 faecal E. coli isolates was constituted from several animal popu...
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Published: |
2006
|
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.5681 http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.1073.5681 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.1073.5681 2023-05-15T14:04:20+02:00 Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. David Skurnik Raymond Ruimy Antoine Andremont Christine Amorin Pierre Rouquet Bertrand Picard Erick Denamur The Pennsylvania State University CiteSeerX Archives 2006 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.5681 http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.5681 http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf text 2006 ftciteseerx 2020-04-26T00:26:11Z Objectives: To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods: A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515-18). Integrons were characterized by triplex realtime PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results: A gradient of resistance ranging from absence to high prevalence (resistance score of 18.7%) and a gradual increase in the prevalence of class 1 integrons (from 0% to 16%), both correlated with the increase in exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions: Our findings firmly establish that the current prevalence of antimicrobial resistance found in animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. Text Antarc* Antarctic Unknown Antarctic The Antarctic |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
Objectives: To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods: A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515-18). Integrons were characterized by triplex realtime PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results: A gradient of resistance ranging from absence to high prevalence (resistance score of 18.7%) and a gradual increase in the prevalence of class 1 integrons (from 0% to 16%), both correlated with the increase in exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions: Our findings firmly establish that the current prevalence of antimicrobial resistance found in animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
David Skurnik Raymond Ruimy Antoine Andremont Christine Amorin Pierre Rouquet Bertrand Picard Erick Denamur |
spellingShingle |
David Skurnik Raymond Ruimy Antoine Andremont Christine Amorin Pierre Rouquet Bertrand Picard Erick Denamur Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
author_facet |
David Skurnik Raymond Ruimy Antoine Andremont Christine Amorin Pierre Rouquet Bertrand Picard Erick Denamur |
author_sort |
David Skurnik |
title |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
title_short |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
title_full |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
title_fullStr |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
title_full_unstemmed |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli”. |
title_sort |
effect of human vicinity on antimicrobial resistance and integrons in animal faecal escherichia coli”. |
publishDate |
2006 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.5681 http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1073.5681 http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/jac2006.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766275387728330752 |