Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing
Abstract Protists in high-latitude lakes are constrained by cold temperatures, low inorganic nutrient supply and low light availability for much of the year due to ice cover and polar darkness. The lengthening ice-free periods in these freshwater ecosystems due to a warming climate results in increa...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1069.9318 2023-05-15T14:58:44+02:00 Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing Sophie Charvet Warwick F Vincent Connie Lovejoy The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1069.9318 http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1069.9318 http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf text ftciteseerx 2020-04-26T00:18:26Z Abstract Protists in high-latitude lakes are constrained by cold temperatures, low inorganic nutrient supply and low light availability for much of the year due to ice cover and polar darkness. The lengthening ice-free periods in these freshwater ecosystems due to a warming climate results in increased light availability, but the overall impacts on phytoplankton and other protists are unknown. We experimentally investigated protist community responses to changes in light and prey availability in a dilution series in Ward Hunt Lake (latitude 83°05 0 N), in the Canadian High Arctic. The communities at the end of the experiment were characterized using high-throughput pyrosequencing of the V4 region of the 18S rRNA gene as a measure of taxonomic presence, and of 18S rRNA (from RNA converted to cDNA) as a taxon-specific indicator of community response. At the end of the experiment under low irradiance, cDNA reads were dominated by photosynthetic dinoflagellate genera, except at the greatest dilution where Cercozoa were most abundant. In contrast, the cDNA reads in the high light treatments were dominated by chrysophytes. Given the known trophic differences among dinoflagellates, cercozoans and chrysophytes, this apparent environmental selection implies that the rise in underwater irradiance associated with increasing ice-free conditions may affect microbial food web structure and function in polar lakes. Text Arctic Phytoplankton Unknown Arctic |
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Abstract Protists in high-latitude lakes are constrained by cold temperatures, low inorganic nutrient supply and low light availability for much of the year due to ice cover and polar darkness. The lengthening ice-free periods in these freshwater ecosystems due to a warming climate results in increased light availability, but the overall impacts on phytoplankton and other protists are unknown. We experimentally investigated protist community responses to changes in light and prey availability in a dilution series in Ward Hunt Lake (latitude 83°05 0 N), in the Canadian High Arctic. The communities at the end of the experiment were characterized using high-throughput pyrosequencing of the V4 region of the 18S rRNA gene as a measure of taxonomic presence, and of 18S rRNA (from RNA converted to cDNA) as a taxon-specific indicator of community response. At the end of the experiment under low irradiance, cDNA reads were dominated by photosynthetic dinoflagellate genera, except at the greatest dilution where Cercozoa were most abundant. In contrast, the cDNA reads in the high light treatments were dominated by chrysophytes. Given the known trophic differences among dinoflagellates, cercozoans and chrysophytes, this apparent environmental selection implies that the rise in underwater irradiance associated with increasing ice-free conditions may affect microbial food web structure and function in polar lakes. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Sophie Charvet Warwick F Vincent Connie Lovejoy |
spellingShingle |
Sophie Charvet Warwick F Vincent Connie Lovejoy Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
author_facet |
Sophie Charvet Warwick F Vincent Connie Lovejoy |
author_sort |
Sophie Charvet |
title |
Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
title_short |
Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
title_full |
Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
title_fullStr |
Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
title_full_unstemmed |
Effects of light and prey availability on Arctic freshwater protist communities examined by high-throughput DNA and RNA sequencing |
title_sort |
effects of light and prey availability on arctic freshwater protist communities examined by high-throughput dna and rna sequencing |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1069.9318 http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Phytoplankton |
genre_facet |
Arctic Phytoplankton |
op_source |
http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1069.9318 http://www.cen.ulaval.ca/warwickvincent/PDFfiles/302-Charvet.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766330855816429568 |