Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae
Abstract The commensal bacteria in the intestine play essential roles in the development and functionality of the host. To unravel the host^microbe interactions in Atlantic cod (Gadus morhua L.) larvae, we used two molecular approaches: (1) suppression subtractive hybridizationpolymerase chain react...
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1061.1909 http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.1061.1909 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.1061.1909 2023-05-15T15:27:38+02:00 Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae Torunn Forberg Augustine Arukwe Olav Vadstein The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1061.1909 http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1061.1909 http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf text ftciteseerx 2020-04-19T00:19:36Z Abstract The commensal bacteria in the intestine play essential roles in the development and functionality of the host. To unravel the host^microbe interactions in Atlantic cod (Gadus morhua L.) larvae, we used two molecular approaches: (1) suppression subtractive hybridizationpolymerase chain reaction (SSH-PCR) to identify host gene responses and (2) expression analysis of selected genes reported to be di¡erentially expressed in gnotobiotic zebra¢sh in a previous study to determine whether these host responses are also conserved in cod. Suppression subtractive hybridization-PCR identi¢ed 156 transcripts putatively regulated by the presence of bacteria. However, out of 22 selected transcripts, only four were signi¢cantly di¡erentially expressed when quanti¢ed using quantitative (real-time) PCR. Expression analysis of selected genes from zebra¢sh revealed possible conservation of host responses for three out of eight genes analysed. For most of the genes quanti¢ed, the gene expression pattern varied between two biological replicates. This may re£ect differences in the bacterial composition in the rearing bottles, and denaturing gradient gel electrophoresis analysis con¢rmed signi¢cant di¡erences between the two replicates with regard to bacterial diversity. The varying e¡ects on gene expression caused by di¡er-ences in the microbial composition show the necessity of further studies where axenic cod larvae are compared with larvae raised in de¢ned and controlled (gnotobiotic) environments. Text atlantic cod Gadus morhua Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
Abstract The commensal bacteria in the intestine play essential roles in the development and functionality of the host. To unravel the host^microbe interactions in Atlantic cod (Gadus morhua L.) larvae, we used two molecular approaches: (1) suppression subtractive hybridizationpolymerase chain reaction (SSH-PCR) to identify host gene responses and (2) expression analysis of selected genes reported to be di¡erentially expressed in gnotobiotic zebra¢sh in a previous study to determine whether these host responses are also conserved in cod. Suppression subtractive hybridization-PCR identi¢ed 156 transcripts putatively regulated by the presence of bacteria. However, out of 22 selected transcripts, only four were signi¢cantly di¡erentially expressed when quanti¢ed using quantitative (real-time) PCR. Expression analysis of selected genes from zebra¢sh revealed possible conservation of host responses for three out of eight genes analysed. For most of the genes quanti¢ed, the gene expression pattern varied between two biological replicates. This may re£ect differences in the bacterial composition in the rearing bottles, and denaturing gradient gel electrophoresis analysis con¢rmed signi¢cant di¡erences between the two replicates with regard to bacterial diversity. The varying e¡ects on gene expression caused by di¡er-ences in the microbial composition show the necessity of further studies where axenic cod larvae are compared with larvae raised in de¢ned and controlled (gnotobiotic) environments. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Torunn Forberg Augustine Arukwe Olav Vadstein |
spellingShingle |
Torunn Forberg Augustine Arukwe Olav Vadstein Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
author_facet |
Torunn Forberg Augustine Arukwe Olav Vadstein |
author_sort |
Torunn Forberg |
title |
Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
title_short |
Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
title_full |
Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
title_fullStr |
Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
title_full_unstemmed |
Two strategies to unravel gene expression responses of host-microbe interactions in cod (Gadus morhua) larvae |
title_sort |
two strategies to unravel gene expression responses of host-microbe interactions in cod (gadus morhua) larvae |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1061.1909 http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1061.1909 http://www.promicrobe.ugent.be/Public/Publications/Papers/Forberg_et_al_2011_Two_strategies.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766358053340315648 |