Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155
Abstract Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separate...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1053.7357 2023-05-15T13:50:42+02:00 Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 Elke De Clerck Dirk Gevers Kjell Sergeant c Marina Rodríguez-Díaz Lieve Herman Niall A Logan Jozef Van Beeumen Paul De Vos The Pennsylvania State University CiteSeerX Archives 2004 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1053.7357 http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1053.7357 http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf text 2004 ftciteseerx 2020-04-12T00:18:45Z Abstract Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share similar temperature and pH conditions, but have substantially different organic loads. Because of the prevalence in gelatine extracts and the dissimilarity of this habitat to geothermal soil, a comparative study was performed to assess the diversity among B. fumarioli strains and reveal possible intraspecies differences that might correspond to their niches of origin. Genomic (rep-PCR, 16S rDNA sequencing, DNA-DNA hybridisations) and phenotypic techniques (analysis of fatty acid content, total cellular proteins, metabolic and morphological traits) illustrate the very close relationship between isolates from the two niches. An abundant protein band was demonstrated for gelatine isolates only. This band was shown to result from a protein with high similarity to a stress response protein. Furthermore, subtractive hybridisation revealed genomic differences between Antarctic and gelatine isolates that may indicate adaptive evolution to a specific environment. 2004 Elsevier SAS. All rights reserved. Text Antarc* Antarctic Antarctica Unknown Antarctic |
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description |
Abstract Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share similar temperature and pH conditions, but have substantially different organic loads. Because of the prevalence in gelatine extracts and the dissimilarity of this habitat to geothermal soil, a comparative study was performed to assess the diversity among B. fumarioli strains and reveal possible intraspecies differences that might correspond to their niches of origin. Genomic (rep-PCR, 16S rDNA sequencing, DNA-DNA hybridisations) and phenotypic techniques (analysis of fatty acid content, total cellular proteins, metabolic and morphological traits) illustrate the very close relationship between isolates from the two niches. An abundant protein band was demonstrated for gelatine isolates only. This band was shown to result from a protein with high similarity to a stress response protein. Furthermore, subtractive hybridisation revealed genomic differences between Antarctic and gelatine isolates that may indicate adaptive evolution to a specific environment. 2004 Elsevier SAS. All rights reserved. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Elke De Clerck Dirk Gevers Kjell Sergeant c Marina Rodríguez-Díaz Lieve Herman Niall A Logan Jozef Van Beeumen Paul De Vos |
spellingShingle |
Elke De Clerck Dirk Gevers Kjell Sergeant c Marina Rodríguez-Díaz Lieve Herman Niall A Logan Jozef Van Beeumen Paul De Vos Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
author_facet |
Elke De Clerck Dirk Gevers Kjell Sergeant c Marina Rodríguez-Díaz Lieve Herman Niall A Logan Jozef Van Beeumen Paul De Vos |
author_sort |
Elke De Clerck |
title |
Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
title_short |
Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
title_full |
Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
title_fullStr |
Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
title_full_unstemmed |
Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine. Research in Microbiology 155 |
title_sort |
genomic and phenotypic comparison of bacillus fumarioli isolates from geothermal antarctic soil and gelatine. research in microbiology 155 |
publishDate |
2004 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1053.7357 http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1053.7357 http://bioinformatics.psb.ugent.be/pdf/science_digev_fumaroli.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766253881856098304 |