Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178
Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1038.5421 2023-05-15T13:34:24+02:00 Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 Quanfu Wang Yanhua Hou Yonglei Shi Xiao Han Qian Chen Zhiguo Hu Yuanping Liu Yujin Li The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1038.5421 http://downloads.hindawi.com/journals/bmri/2014/246871.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1038.5421 http://downloads.hindawi.com/journals/bmri/2014/246871.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://downloads.hindawi.com/journals/bmri/2014/246871.pdf text ftciteseerx 2020-03-08T01:19:07Z Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa. Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC. Recombinant PsGrx (rPsGrx) stably expressed in E. coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography. rPsGrx exhibited optimal activity at 30 ∘ C and pH 8.0 and showed 25.5% of the activity at 0 ∘ C. It retained 65.0% of activity after incubation at 40 ∘ C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl. These results indicated that rPsGrx was a typical cold active protein with low thermostability. Text Antarc* Antarctic Sea ice Unknown Antarctic |
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ftciteseerx |
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English |
description |
Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide. In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp. AN178. It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa. Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC. Recombinant PsGrx (rPsGrx) stably expressed in E. coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography. rPsGrx exhibited optimal activity at 30 ∘ C and pH 8.0 and showed 25.5% of the activity at 0 ∘ C. It retained 65.0% of activity after incubation at 40 ∘ C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl. These results indicated that rPsGrx was a typical cold active protein with low thermostability. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Quanfu Wang Yanhua Hou Yonglei Shi Xiao Han Qian Chen Zhiguo Hu Yuanping Liu Yujin Li |
spellingShingle |
Quanfu Wang Yanhua Hou Yonglei Shi Xiao Han Qian Chen Zhiguo Hu Yuanping Liu Yujin Li Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
author_facet |
Quanfu Wang Yanhua Hou Yonglei Shi Xiao Han Qian Chen Zhiguo Hu Yuanping Liu Yujin Li |
author_sort |
Quanfu Wang |
title |
Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
title_short |
Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
title_full |
Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
title_fullStr |
Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
title_full_unstemmed |
Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178 |
title_sort |
characterization of glutaredoxin from antarctic sea-ice bacterium pseudoalteromonas sp. an178 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1038.5421 http://downloads.hindawi.com/journals/bmri/2014/246871.pdf |
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Antarctic |
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Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_source |
http://downloads.hindawi.com/journals/bmri/2014/246871.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1038.5421 http://downloads.hindawi.com/journals/bmri/2014/246871.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766052398047952896 |