Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas

• Main Authors: Xiaotong Wang, Li Li, Fei Xu, Guofan Zhang
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Pacific; Crassostrea gigas; Pacific oyster
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1037.412; http://cmsadmin.atp.co.il/Content_siamb/editor/63.2011.671.Zhang.pdf

Abstract As opposed to other animals, live bivalve shellfish cannot be sampled because of the high mortality associated with opening closed shells. In this paper, we present a new anesthesia method using an MgSO 4 seawater solution for sampling live bivalve shellfish. In our experiment, genomic DNA was successfully extracted from mantle tissues of sampled Pacific oyster (Crassostrea gigas) and the DNA was used as a template for polymerase chain reaction (PCR), which indicated that 3 mm 3 of mantle tissue was enough for DNA extraction and subsequent molecular biology testing. The survival rate after the in vivo sampling was high (83.3%), indicating that the sampling method caused only slight harm to the oysters. Thus, MgSO 4 anesthesia is a practical method for in vivo sampling of oysters and probably could be applied to other bivalve shellfish species as well, significantly promoting the application of molecular marker-assisted selection in bivalve shellfish breeding and the study of tissue injury and repair mechanisms in bivalve shellfish.