Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase
The gastrointestinal tract of animals is adapted to their primary source of food to optimize resource use and energy intake. Temperate bat species mainly feed on arthropods. These contain the energy-rich carbohydrate chitin, which is indigestible for the endogenous enzymes of a typical mammalian gas...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.1032.7263 2023-05-15T17:13:46+02:00 Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase Sara Strobel Anna Roswag Nina I. Becker Tina E. Trenczek Jorge A. Encarnação The Pennsylvania State University CiteSeerX Archives http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1032.7263 http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1032.7263 http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF text ftciteseerx 2016-10-30T00:15:24Z The gastrointestinal tract of animals is adapted to their primary source of food to optimize resource use and energy intake. Temperate bat species mainly feed on arthropods. These contain the energy-rich carbohydrate chitin, which is indigestible for the endogenous enzymes of a typical mammalian gastrointestinal tract. However, the gastrointestinal tract of bat species should be adapted to their diet and be able to digest chitin. We hypothesized that (i) European vespertilionid bat species have the digestive enzyme chitinase and that (ii) the chitinolytic activity is located in the intestine, as has been found for North American bat species. The gastrointestinal tracts of seven bat species (Pipistrellus pipistrellus, Plecotus auritus, Myotis bechsteinii, Myotis nattereri, Myotis daubentonii, Myotis myotis, and Nyctalus leisleri) were tested for chitinolytic activity by diffusion assay. Gastrointestinal tracts of P. pipistrellus, P. auritus, M. nattereri, M. myotis, and N. leisleri were examined for acidic mammalian chitinase by western blot analysis. Tissue sections of the gastrointestinal tract of P. pipistrellus were immunohistochemically analyzed to locate the acidic mammalian chitinase. Chitinolytic activity was detected in the stomachs of all bat species. Western blot analysis confirmed the acidic mammalian chitinase in stomach samples. Immunohistochemistry of the P. pipistrellus gastrointestinal tract indicated that acidic mammalian chitinase is located in the stomach chief cells at the base of the gastric glands. In conclusion, European vespertilionid bat species have acidic mammalian chitinase that is produced in the gastric glands of the stomach. Therefore, the gastrointestinal tracts of Text Myotis nattereri Pipistrellus pipistrellus Unknown |
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English |
description |
The gastrointestinal tract of animals is adapted to their primary source of food to optimize resource use and energy intake. Temperate bat species mainly feed on arthropods. These contain the energy-rich carbohydrate chitin, which is indigestible for the endogenous enzymes of a typical mammalian gastrointestinal tract. However, the gastrointestinal tract of bat species should be adapted to their diet and be able to digest chitin. We hypothesized that (i) European vespertilionid bat species have the digestive enzyme chitinase and that (ii) the chitinolytic activity is located in the intestine, as has been found for North American bat species. The gastrointestinal tracts of seven bat species (Pipistrellus pipistrellus, Plecotus auritus, Myotis bechsteinii, Myotis nattereri, Myotis daubentonii, Myotis myotis, and Nyctalus leisleri) were tested for chitinolytic activity by diffusion assay. Gastrointestinal tracts of P. pipistrellus, P. auritus, M. nattereri, M. myotis, and N. leisleri were examined for acidic mammalian chitinase by western blot analysis. Tissue sections of the gastrointestinal tract of P. pipistrellus were immunohistochemically analyzed to locate the acidic mammalian chitinase. Chitinolytic activity was detected in the stomachs of all bat species. Western blot analysis confirmed the acidic mammalian chitinase in stomach samples. Immunohistochemistry of the P. pipistrellus gastrointestinal tract indicated that acidic mammalian chitinase is located in the stomach chief cells at the base of the gastric glands. In conclusion, European vespertilionid bat species have acidic mammalian chitinase that is produced in the gastric glands of the stomach. Therefore, the gastrointestinal tracts of |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Sara Strobel Anna Roswag Nina I. Becker Tina E. Trenczek Jorge A. Encarnação |
spellingShingle |
Sara Strobel Anna Roswag Nina I. Becker Tina E. Trenczek Jorge A. Encarnação Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
author_facet |
Sara Strobel Anna Roswag Nina I. Becker Tina E. Trenczek Jorge A. Encarnação |
author_sort |
Sara Strobel |
title |
Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
title_short |
Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
title_full |
Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
title_fullStr |
Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
title_full_unstemmed |
Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase |
title_sort |
insectivorous bats digest chitin in the stomach using acidic mammalian chitinase |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1032.7263 http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF |
genre |
Myotis nattereri Pipistrellus pipistrellus |
genre_facet |
Myotis nattereri Pipistrellus pipistrellus |
op_source |
http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1032.7263 http://journals.plos.org/plosone/article/asset?id%3D10.1371/journal.pone.0072770.PDF |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766070956400312320 |