Secretion of -Amylase from Pseudoalteromonas haloplanktis TAB23: Two Different Pathways in Different Hosts

• Main Authors: Georges Feller, Gennaro Marino
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 2002
• Subjects: Antarctic; Antarc*
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1027.1996; http://orbi.ulg.ac.be/bitstream/2268/15877/1/JBact_2002_Ct.pdf

Secretion of cold-adapted -amylase from Pseudoalteromonas haloplanktis TAB23 was studied in three Antarctic bacteria. We demonstrated that the enzyme is specifically secreted in the psychrophilic hosts even in the absence of a protein domain that has been previously reported to be necessary for -amylase secretion in Escherichia coli. The occurrence of two different secretion pathways in different hosts is proposed. The presence of an outer membrane in gram-negative bac-teria imposes a barrier for the secretion of proteins into the extracellular medium. At least five different, widely dissemi-nated secretion pathways have evolved in these bacteria for the translocation of soluble proteins across the outer membrane