Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females
International audience The relative roles of genetic (GSD) and temperature sex determination (TSD) were investigated in turbot, Scophthalmus maximus, with the objective of producing all-female stocks for aquaculture. Androgen treated males (ATM) and oestrogen treated females (OTF) were produced afte...
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Main Authors: | , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2009
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Online Access: | https://hal.inrae.fr/hal-02668561 https://doi.org/10.1016/j.aquaculture.2009.05.004 |
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ftciradhal:oai:HAL:hal-02668561v1 2023-12-24T10:24:41+01:00 Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females Haffray, Pierrick Lebègue, Elisabeth Jeu, Sylvie Guennoc, Michel Guiguen, Yann Baroiller, Jean-François Fostier, Alexis Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) France Turbot - Marinove Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) 2009 https://hal.inrae.fr/hal-02668561 https://doi.org/10.1016/j.aquaculture.2009.05.004 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2009.05.004 hal-02668561 https://hal.inrae.fr/hal-02668561 doi:10.1016/j.aquaculture.2009.05.004 PRODINRA: 26898 WOS: 000268950700006 ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.inrae.fr/hal-02668561 Aquaculture, 2009, 294 (1-2), pp.30-36. ⟨10.1016/j.aquaculture.2009.05.004⟩ http://www.journals.elsevier.com/aquaculture/ all-female zz/zw sex determination GSD TSD feminization genetique animale population monosexe [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology info:eu-repo/semantics/article Journal articles 2009 ftciradhal https://doi.org/10.1016/j.aquaculture.2009.05.004 2023-11-29T17:27:16Z International audience The relative roles of genetic (GSD) and temperature sex determination (TSD) were investigated in turbot, Scophthalmus maximus, with the objective of producing all-female stocks for aquaculture. Androgen treated males (ATM) and oestrogen treated females (OTF) were produced after testing various 17 alpha-methyltestosterone or 17 beta-oestradiol treatments delivered orally (3 or 5 mg steroids/kg food) from 35 days postfertilization (dpf) during either 500 or 700 degree-days respectively. GSD was analyzed by checking the sex ratio of families (n = 33) obtained by crossing control parents with other control parents, either ATM or OTF. TSD was investigated on 8 families reared at three temperatures (15 degrees C, 17 degrees C or 24 degrees C) from weaning to 100 dpf. All the steroid treatments were efficient in producing either 100% mate or 100% female populations demonstrating the production of androgen sex-inversed males and oestrogen sex-inversed females. The results of the GSD analysis suggested that most of the data fit to a ZZ/ZW model of sex determination. However, some crosses deviated from this model and other minor factors may be involved. interestingly, minor effects of temperature and a limited but significant interaction between family and temperature have been observed. In conclusion, the present data suggest that a major ZZ/ZW genetic model is involved in turbot sex determination. Unexpected sex ratios deviating from this model are discussed, including a possible minor effect of environmental factors, such as temperature. (C) 2009 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Scophthalmus maximus Turbot CIRAD: HAL (Agricultural Research for Development) Aquaculture 294 1-2 30 36 |
institution |
Open Polar |
collection |
CIRAD: HAL (Agricultural Research for Development) |
op_collection_id |
ftciradhal |
language |
English |
topic |
all-female zz/zw sex determination GSD TSD feminization genetique animale population monosexe [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
spellingShingle |
all-female zz/zw sex determination GSD TSD feminization genetique animale population monosexe [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Haffray, Pierrick Lebègue, Elisabeth Jeu, Sylvie Guennoc, Michel Guiguen, Yann Baroiller, Jean-François Fostier, Alexis Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
topic_facet |
all-female zz/zw sex determination GSD TSD feminization genetique animale population monosexe [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology |
description |
International audience The relative roles of genetic (GSD) and temperature sex determination (TSD) were investigated in turbot, Scophthalmus maximus, with the objective of producing all-female stocks for aquaculture. Androgen treated males (ATM) and oestrogen treated females (OTF) were produced after testing various 17 alpha-methyltestosterone or 17 beta-oestradiol treatments delivered orally (3 or 5 mg steroids/kg food) from 35 days postfertilization (dpf) during either 500 or 700 degree-days respectively. GSD was analyzed by checking the sex ratio of families (n = 33) obtained by crossing control parents with other control parents, either ATM or OTF. TSD was investigated on 8 families reared at three temperatures (15 degrees C, 17 degrees C or 24 degrees C) from weaning to 100 dpf. All the steroid treatments were efficient in producing either 100% mate or 100% female populations demonstrating the production of androgen sex-inversed males and oestrogen sex-inversed females. The results of the GSD analysis suggested that most of the data fit to a ZZ/ZW model of sex determination. However, some crosses deviated from this model and other minor factors may be involved. interestingly, minor effects of temperature and a limited but significant interaction between family and temperature have been observed. In conclusion, the present data suggest that a major ZZ/ZW genetic model is involved in turbot sex determination. Unexpected sex ratios deviating from this model are discussed, including a possible minor effect of environmental factors, such as temperature. (C) 2009 Elsevier B.V. All rights reserved. |
author2 |
Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF) France Turbot - Marinove Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
format |
Article in Journal/Newspaper |
author |
Haffray, Pierrick Lebègue, Elisabeth Jeu, Sylvie Guennoc, Michel Guiguen, Yann Baroiller, Jean-François Fostier, Alexis |
author_facet |
Haffray, Pierrick Lebègue, Elisabeth Jeu, Sylvie Guennoc, Michel Guiguen, Yann Baroiller, Jean-François Fostier, Alexis |
author_sort |
Haffray, Pierrick |
title |
Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
title_short |
Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
title_full |
Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
title_fullStr |
Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
title_full_unstemmed |
Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation: An investigation using steroid sex-inverted males and females |
title_sort |
genetic determination and temperature effects on turbot scophthalmus maximus sex differentiation: an investigation using steroid sex-inverted males and females |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.inrae.fr/hal-02668561 https://doi.org/10.1016/j.aquaculture.2009.05.004 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.inrae.fr/hal-02668561 Aquaculture, 2009, 294 (1-2), pp.30-36. ⟨10.1016/j.aquaculture.2009.05.004⟩ http://www.journals.elsevier.com/aquaculture/ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2009.05.004 hal-02668561 https://hal.inrae.fr/hal-02668561 doi:10.1016/j.aquaculture.2009.05.004 PRODINRA: 26898 WOS: 000268950700006 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2009.05.004 |
container_title |
Aquaculture |
container_volume |
294 |
container_issue |
1-2 |
container_start_page |
30 |
op_container_end_page |
36 |
_version_ |
1786199763418349568 |