Polymorphic microsatellite loci and PCR multiplexing in the common vole, Microtus arvalis

• Published in: Molecular Ecology Notes
• Main Authors: Gauffre, Bertrand, Galan, Maxime, Bretagnolle, V., Cosson, Jean-Francois, J.-F.
• Other Authors: Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre d'Études Biologiques de Chizé (CEBC), Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2007
• Subjects: MICROTUS ARVALIS; MULTIPLEX; RODENTIA; CAMPAGNOL DES CHAMPS; COMMON VOLE; MICROSATELLITE; GENETIQUE DES POPULATIONS; [SDV.EE]Life Sciences [q-bio]/Ecology; environment; Common vole; Microtus arvalis
• Online Access: https://hal.inrae.fr/hal-02663395; https://doi.org/10.1111/j.1471-8286.2007.01718.x

International audience We isolated and characterized 14 dinucleotide microsatellite loci in the common vole Microtus arvalis (Palas). Two multiplex panels both comprising seven loci were developed. Application to a set of 21 individuals allowed clear and easy characterization of allele sizes except for two loci which were then withdrawn from further analyses. The number of alleles ranged from four to 19 per locus with the observed heterozygosity ranging from 0.55 to 0.95. These sets of microsatellite loci provide high throughput capacity for population genetic studies at a minimum cost