Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts
Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distribution includ...
Published in: | mSystems |
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Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2021
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Subjects: | |
Online Access: | http://agritrop.cirad.fr/607097/ http://agritrop.cirad.fr/607097/1/607097.pdf https://doi.org/10.1128/mSystems.00258-21 |
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ftcirad:oai:agritrop.cirad.fr:607097 |
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openpolar |
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Open Polar |
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CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) |
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ftcirad |
language |
English |
topic |
phylogénie génome écotype phénotype tolérance au sel Moraxella micro-organisme psychrophile croissance séquence nucléotidique écologie animale aptitude à coloniser génie génétique sel biliaire glace d'eau de mer http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4934 http://aims.fao.org/aos/agrovoc/c_24069 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_36703 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_905 http://aims.fao.org/aos/agrovoc/c_25108e09 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
phylogénie génome écotype phénotype tolérance au sel Moraxella micro-organisme psychrophile croissance séquence nucléotidique écologie animale aptitude à coloniser génie génétique sel biliaire glace d'eau de mer http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4934 http://aims.fao.org/aos/agrovoc/c_24069 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_36703 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_905 http://aims.fao.org/aos/agrovoc/c_25108e09 http://aims.fao.org/aos/agrovoc/c_3081 Welter, Daphne K. Ruaud, Albane Henseler, Zachariah M. De Jong, Hannah N. Van Coeverden de Groot, Peter Michaux, Johan Gormezano, Linda Waters, Jillian L. Youngblut, Nicholas D. Ley, Ruth E. Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
topic_facet |
phylogénie génome écotype phénotype tolérance au sel Moraxella micro-organisme psychrophile croissance séquence nucléotidique écologie animale aptitude à coloniser génie génétique sel biliaire glace d'eau de mer http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4934 http://aims.fao.org/aos/agrovoc/c_24069 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_36703 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_905 http://aims.fao.org/aos/agrovoc/c_25108e09 http://aims.fao.org/aos/agrovoc/c_3081 |
description |
Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distribution including animal bodies as well as sea ice and other nonhost environments. To elucidate the relationship between these ecological niches and Psychrobacter's evolutionary history, we performed tandem genomic analyses with phenotyping of 85 Psychrobacter accessions. Phylogenomic analysis of the family Moraxellaceae reveals that basal members of the Psychrobacter clade are Moraxella spp., a group of often-pathogenic organisms. Psychrobacter exhibited two broad growth patterns in our phenotypic screen: one group that we called the “flexible ecotype” (FE) had the ability to grow between 4 and 37°C, and the other, which we called the “restricted ecotype” (RE), could grow between 4 and 25°C. The FE group includes phylogenetically basal strains, and FE strains exhibit increased transposon copy numbers, smaller genomes, and a higher likelihood to be bile salt resistant. The RE group contains only phylogenetically derived strains and has increased proportions of lipid metabolism and biofilm formation genes, functions that are adaptive to cold stress. In a 16S rRNA gene survey of polar bear fecal samples, we detect both FE and RE strains, but in in vivo colonizations of gnotobiotic mice, only FE strains persist. Our results indicate the ability to grow at 37°C, seemingly necessary for mammalian gut colonization, is an ancestral trait for Psychrobacter, which likely evolved from a pathobiont. IMPORTANCE Host-associated microbes are generally assumed to have evolved from free-living ones. The evolutionary transition of microbes in the opposite direction, from host associated toward free living, has been predicted based on phylogenetic data but not studied in depth. Here, we provide evidence that the genus Psychrobacter, particularly ... |
format |
Article in Journal/Newspaper |
author |
Welter, Daphne K. Ruaud, Albane Henseler, Zachariah M. De Jong, Hannah N. Van Coeverden de Groot, Peter Michaux, Johan Gormezano, Linda Waters, Jillian L. Youngblut, Nicholas D. Ley, Ruth E. |
author_facet |
Welter, Daphne K. Ruaud, Albane Henseler, Zachariah M. De Jong, Hannah N. Van Coeverden de Groot, Peter Michaux, Johan Gormezano, Linda Waters, Jillian L. Youngblut, Nicholas D. Ley, Ruth E. |
author_sort |
Welter, Daphne K. |
title |
Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
title_short |
Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
title_full |
Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
title_fullStr |
Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
title_full_unstemmed |
Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts |
title_sort |
free-living, psychrotrophic bacteria of the genus psychrobacter are descendants of pathobionts |
publishDate |
2021 |
url |
http://agritrop.cirad.fr/607097/ http://agritrop.cirad.fr/607097/1/607097.pdf https://doi.org/10.1128/mSystems.00258-21 |
op_coverage |
France |
genre |
polar bear Sea ice |
genre_facet |
polar bear Sea ice |
op_source |
mSystems |
op_relation |
http://agritrop.cirad.fr/607097/ Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts. Welter Daphne K., Ruaud Albane, Henseler Zachariah M., De Jong Hannah N., Van Coeverden de Groot Peter, Michaux Johan, Gormezano Linda, Waters Jillian L., Youngblut Nicholas D., Ley Ruth E. 2021. mSystems, 6 (2), 20 p.https://doi.org/10.1128/mSystems.00258-21 <https://doi.org/10.1128/mSystems.00258-21> http://agritrop.cirad.fr/607097/1/607097.pdf |
op_rights |
cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1128/mSystems.00258-21 |
container_title |
mSystems |
container_volume |
6 |
container_issue |
2 |
_version_ |
1799487977068429312 |
spelling |
ftcirad:oai:agritrop.cirad.fr:607097 2024-05-19T07:47:33+00:00 Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts Welter, Daphne K. Ruaud, Albane Henseler, Zachariah M. De Jong, Hannah N. Van Coeverden de Groot, Peter Michaux, Johan Gormezano, Linda Waters, Jillian L. Youngblut, Nicholas D. Ley, Ruth E. France 2021 text http://agritrop.cirad.fr/607097/ http://agritrop.cirad.fr/607097/1/607097.pdf https://doi.org/10.1128/mSystems.00258-21 eng eng http://agritrop.cirad.fr/607097/ Free-living, psychrotrophic bacteria of the Genus Psychrobacter are descendants of pathobionts. Welter Daphne K., Ruaud Albane, Henseler Zachariah M., De Jong Hannah N., Van Coeverden de Groot Peter, Michaux Johan, Gormezano Linda, Waters Jillian L., Youngblut Nicholas D., Ley Ruth E. 2021. mSystems, 6 (2), 20 p.https://doi.org/10.1128/mSystems.00258-21 <https://doi.org/10.1128/mSystems.00258-21> http://agritrop.cirad.fr/607097/1/607097.pdf cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ mSystems phylogénie génome écotype phénotype tolérance au sel Moraxella micro-organisme psychrophile croissance séquence nucléotidique écologie animale aptitude à coloniser génie génétique sel biliaire glace d'eau de mer http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4934 http://aims.fao.org/aos/agrovoc/c_24069 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_36703 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_905 http://aims.fao.org/aos/agrovoc/c_25108e09 http://aims.fao.org/aos/agrovoc/c_3081 article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion 2021 ftcirad https://doi.org/10.1128/mSystems.00258-21 2024-05-02T00:07:14Z Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum Gammaproteobacteria, family Moraxellaceae, the genus Psychrobacter includes strains from a broad ecological distribution including animal bodies as well as sea ice and other nonhost environments. To elucidate the relationship between these ecological niches and Psychrobacter's evolutionary history, we performed tandem genomic analyses with phenotyping of 85 Psychrobacter accessions. Phylogenomic analysis of the family Moraxellaceae reveals that basal members of the Psychrobacter clade are Moraxella spp., a group of often-pathogenic organisms. Psychrobacter exhibited two broad growth patterns in our phenotypic screen: one group that we called the “flexible ecotype” (FE) had the ability to grow between 4 and 37°C, and the other, which we called the “restricted ecotype” (RE), could grow between 4 and 25°C. The FE group includes phylogenetically basal strains, and FE strains exhibit increased transposon copy numbers, smaller genomes, and a higher likelihood to be bile salt resistant. The RE group contains only phylogenetically derived strains and has increased proportions of lipid metabolism and biofilm formation genes, functions that are adaptive to cold stress. In a 16S rRNA gene survey of polar bear fecal samples, we detect both FE and RE strains, but in in vivo colonizations of gnotobiotic mice, only FE strains persist. Our results indicate the ability to grow at 37°C, seemingly necessary for mammalian gut colonization, is an ancestral trait for Psychrobacter, which likely evolved from a pathobiont. IMPORTANCE Host-associated microbes are generally assumed to have evolved from free-living ones. The evolutionary transition of microbes in the opposite direction, from host associated toward free living, has been predicted based on phylogenetic data but not studied in depth. Here, we provide evidence that the genus Psychrobacter, particularly ... Article in Journal/Newspaper polar bear Sea ice CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) mSystems 6 2 |