Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions
This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as 'green solvents' for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence...
Published in: | Process Biochemistry |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2012
|
Subjects: | |
Online Access: | http://agritrop.cirad.fr/566609/ http://agritrop.cirad.fr/566609/1/document_566609.pdf https://doi.org/10.1016/j.procbio.2012.07.027 |
id |
ftcirad:oai:agritrop.cirad.fr:566609 |
---|---|
record_format |
openpolar |
spelling |
ftcirad:oai:agritrop.cirad.fr:566609 2023-05-15T13:54:33+02:00 Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions Durand, Erwann Lecomte, Jérôme Baréa, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre 2012 application/pdf http://agritrop.cirad.fr/566609/ http://agritrop.cirad.fr/566609/1/document_566609.pdf https://doi.org/10.1016/j.procbio.2012.07.027 eng eng http://agritrop.cirad.fr/566609/ Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions. Durand Erwann, Lecomte Jérôme, Baréa Bruno, Piombo Georges, Dubreucq Eric, Villeneuve Pierre. 2012. Process Biochemistry (1991), 47 (12) : 2081-2089.https://doi.org/10.1016/j.procbio.2012.07.027 <https://doi.org/10.1016/j.procbio.2012.07.027> http://agritrop.cirad.fr/566609/1/document_566609.pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html Process Biochemistry (1991) 000 - Autres thèmes Q02 - Traitement et conservation des produits alimentaires Candida Biocatalyseur Estérification Solvant Propriété physicochimique Estérase Lipase http://aims.fao.org/aos/agrovoc/c_1247 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_12194 article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion 2012 ftcirad https://doi.org/10.1016/j.procbio.2012.07.027 2023-01-03T23:49:29Z This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as 'green solvents' for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substrate polarity was assessed using alcohols of various chain lengths. Results showed that grinding of immobilized lipase was essential parameters for good lipase activity. Moreover, in our model reaction some hydrogen-bond donor component from the DES can compete with the alcoholysis reaction. Indeed, side reactions were observed with DES based on dicarboxylic acid or ethylene glycol, leading to some limitations of their use. However, the results showed that other DESs such as choline chloride:urea and choline chloride:glycerol could exhibit high activity and selectivity making them promising solvents for lipase-catalyzed reactions. Finally, the best DES's specific activity - and stability up to five days incubation time - were analyzed and compared with conventional organic solvents. Experiments revealed that iCALB is less influenced by the chain length of alcohol in DES than organic solvents and it is preserves its activity with minimally destructive to protein structure. Article in Journal/Newspaper Antarc* Antarctica CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) Process Biochemistry 47 12 2081 2089 |
institution |
Open Polar |
collection |
CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) |
op_collection_id |
ftcirad |
language |
English |
topic |
000 - Autres thèmes Q02 - Traitement et conservation des produits alimentaires Candida Biocatalyseur Estérification Solvant Propriété physicochimique Estérase Lipase http://aims.fao.org/aos/agrovoc/c_1247 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_12194 |
spellingShingle |
000 - Autres thèmes Q02 - Traitement et conservation des produits alimentaires Candida Biocatalyseur Estérification Solvant Propriété physicochimique Estérase Lipase http://aims.fao.org/aos/agrovoc/c_1247 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_12194 Durand, Erwann Lecomte, Jérôme Baréa, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
topic_facet |
000 - Autres thèmes Q02 - Traitement et conservation des produits alimentaires Candida Biocatalyseur Estérification Solvant Propriété physicochimique Estérase Lipase http://aims.fao.org/aos/agrovoc/c_1247 http://aims.fao.org/aos/agrovoc/c_27460 http://aims.fao.org/aos/agrovoc/c_28361 http://aims.fao.org/aos/agrovoc/c_7236 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_2670 http://aims.fao.org/aos/agrovoc/c_12194 |
description |
This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as 'green solvents' for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substrate polarity was assessed using alcohols of various chain lengths. Results showed that grinding of immobilized lipase was essential parameters for good lipase activity. Moreover, in our model reaction some hydrogen-bond donor component from the DES can compete with the alcoholysis reaction. Indeed, side reactions were observed with DES based on dicarboxylic acid or ethylene glycol, leading to some limitations of their use. However, the results showed that other DESs such as choline chloride:urea and choline chloride:glycerol could exhibit high activity and selectivity making them promising solvents for lipase-catalyzed reactions. Finally, the best DES's specific activity - and stability up to five days incubation time - were analyzed and compared with conventional organic solvents. Experiments revealed that iCALB is less influenced by the chain length of alcohol in DES than organic solvents and it is preserves its activity with minimally destructive to protein structure. |
format |
Article in Journal/Newspaper |
author |
Durand, Erwann Lecomte, Jérôme Baréa, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre |
author_facet |
Durand, Erwann Lecomte, Jérôme Baréa, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre |
author_sort |
Durand, Erwann |
title |
Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
title_short |
Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
title_full |
Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
title_fullStr |
Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
title_full_unstemmed |
Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions |
title_sort |
evaluation of deep eutectic solvents as new media for candida antarctica b lipase catalyzed reactions |
publishDate |
2012 |
url |
http://agritrop.cirad.fr/566609/ http://agritrop.cirad.fr/566609/1/document_566609.pdf https://doi.org/10.1016/j.procbio.2012.07.027 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Process Biochemistry (1991) |
op_relation |
http://agritrop.cirad.fr/566609/ Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions. Durand Erwann, Lecomte Jérôme, Baréa Bruno, Piombo Georges, Dubreucq Eric, Villeneuve Pierre. 2012. Process Biochemistry (1991), 47 (12) : 2081-2089.https://doi.org/10.1016/j.procbio.2012.07.027 <https://doi.org/10.1016/j.procbio.2012.07.027> http://agritrop.cirad.fr/566609/1/document_566609.pdf |
op_rights |
Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
op_doi |
https://doi.org/10.1016/j.procbio.2012.07.027 |
container_title |
Process Biochemistry |
container_volume |
47 |
container_issue |
12 |
container_start_page |
2081 |
op_container_end_page |
2089 |
_version_ |
1766260534516121600 |