Enzymatic production of conjugated linoleic acid monoacylglycerols from dehydrated isomerized castor bean oil

• Published in: Journal of Food Lipids
• Main Authors: Barouh, Nathalie, Piombo, Georges, Goli, Thierry, Baréa, Bruno, Pina, Michel, Lago, Regina C.A., Villeneuve, Pierre
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2008
• Subjects: Q02 - Traitement et conservation des produits alimentaires; Q60 - Traitement des produits agricoles non alimentaires; Huile de ricin; Ricinus communis; Acide linoléique; Biosynthèse; Triacylglycérol lipase; Triglycéride; Candida; Acylglycérole; Activité enzymatique; Isomérisation; Séchage; http://aims.fao.org/aos/agrovoc/c_25466; http://aims.fao.org/aos/agrovoc/c_6600; http://aims.fao.org/aos/agrovoc/c_4352; http://aims.fao.org/aos/agrovoc/c_928; http://aims.fao.org/aos/agrovoc/c_24095; http://aims.fao.org/aos/agrovoc/c_14992; http://aims.fao.org/aos/agrovoc/c_1247; http://aims.fao.org/aos/agrovoc/c_3297; http://aims.fao.org/aos/agrovoc/c_2604; http://aims.fao.org/aos/agrovoc/c_27561; http://aims.fao.org/aos/agrovoc/c_2402; Antarc*; Antarctica
• Online Access: http://agritrop.cirad.fr/543238/; http://agritrop.cirad.fr/543238/1/document_543238.pdf; https://doi.org/10.1111/j.1745-4522.2007.00099.x

The goal of this study was to produce conjugated linoleic acid (CLA) monoacylglycerols (MAGs). A chemicoenzymatic process was employed starting from castor bean oil, which was chemically dehydrated and isomerized to obtain new modified triacylglycerols (TAGs) with a very high proportion of CLA fatty acids (>95%). Such TAGs were then used in enzymatic glycerolysis reaction using Candida antarctica B lipase at 55C in tert-butyl alcohol as a solvent. The effect of enzyme load and substrate molar ratio on the production yield of MAGs was evaluated and optimized using response surface methodology with a two-factor and three-level factorial design. A satisfactory linear correlation existed between experimental results and predicted values (R2 = 0.97 and R2adj. = 0.94). Using the contour plot generated from the model for MAG production yield, optimum parameters could be obtained corresponding to an 11/1 glycerol/TAG molar ratio and an enzyme load of 2% (% w/w of both substrates).