Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels
Several parameters were analyzed to determine the mechanisms responsible for the enhancement of the gill Na+ -K+ -ATPase activity of Atlantic salmon smolts. A major alfa-subunit transcript of 3.7 kb was revealed by Northern blot in both parr and smolt gills when hybridized with two distinct cDNA pro...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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Language: | English |
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Online Access: | http://agritrop.cirad.fr/510591/ http://agritrop.cirad.fr/510591/1/510591.pdf https://doi.org/10.1152/ajpregu.2000.278.1.R101 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=175131 |
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ftcirad:oai:agritrop.cirad.fr:510591 2023-05-15T15:32:10+02:00 Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels D'Cotta, Helena Valotaire, Claudiane Le Gac, Florence Prunet, Patrick 2000 text http://agritrop.cirad.fr/510591/ http://agritrop.cirad.fr/510591/1/510591.pdf https://doi.org/10.1152/ajpregu.2000.278.1.R101 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=175131 eng eng http://agritrop.cirad.fr/510591/ Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels. D'Cotta Helena, Valotaire Claudiane, Le Gac Florence, Prunet Patrick. 2000. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 278 (1), R101-R110https://doi.org/10.1152/ajpregu.2000.278.1.R101 <https://doi.org/10.1152/ajpregu.2000.278.1.R101> http://agritrop.cirad.fr/510591/1/510591.pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html American Journal of Physiology. Regulatory, Integrative and Comparative Physiology L50 - Physiologie et biochimie animales Saumon Sodium Salmo salar Protéine http://aims.fao.org/aos/agrovoc/c_6756 http://aims.fao.org/aos/agrovoc/c_7145 http://aims.fao.org/aos/agrovoc/c_14037 http://aims.fao.org/aos/agrovoc/c_6259 article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion 2000 ftcirad https://doi.org/10.1152/ajpregu.2000.278.1.R101 2022-04-26T22:51:33Z Several parameters were analyzed to determine the mechanisms responsible for the enhancement of the gill Na+ -K+ -ATPase activity of Atlantic salmon smolts. A major alfa-subunit transcript of 3.7 kb was revealed by Northern blot in both parr and smolt gills when hybridized with two distinct cDNA probes. The alfa-mRNA abundance demonstrated an increase to maximal levels in smolts at an early stage of the parr-smolt transformation. This was followed by a gradual rise in alfa-protein levels, revealed by Western blots with specific antibodies and by an increase in gill Na+ -K+ -ATPase hydrolytic activity, both only reaching maximum levels a month later, at the peak of the transformation process. Parr fish experienced a decrease in alfa-mRNA abundance and had basal levels of alfa-protein and enzyme activity. Measurement of the binding of [3H]ouabain to Na+ -K+ -ATPase was characterized in smolts and parr gill membranes showing more than a twofold elevation in smolts and was of high affinity in both groups (dissociation constant = 20-23 nM). Modulation of the enzyme due to increased salinity was also observed in seawater-transferred smolts, as demonstrated by an increase in alfa-mRNA levels after 24 h with a rise in Na+ -K+ -ATPase activity occurring only after 11 days. No qualitative change in alfa-expression was revealed at either the mRNA or protein level. Immunological identification of the alfa-protein was performed with polyclonal antibodies directed against the rat alfa-specific isoforms, revealing that parr, freshwater, and seawater smolts have an alfa3-like isoform. This study shows that the increase in Na+ -K+ -ATPase activity in smolt gills depends first on an increase in the alfa-mRNA expression and is followed by a slower rise in -protein abundance that eventually leads to a higher synthesis of Na+ -K+ pumps. Article in Journal/Newspaper Atlantic salmon Salmo salar CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 278 1 R101 R110 |
institution |
Open Polar |
collection |
CIRAD: Agritrop (Centre de coopération internationale en recherche agronomique pour le développement) |
op_collection_id |
ftcirad |
language |
English |
topic |
L50 - Physiologie et biochimie animales Saumon Sodium Salmo salar Protéine http://aims.fao.org/aos/agrovoc/c_6756 http://aims.fao.org/aos/agrovoc/c_7145 http://aims.fao.org/aos/agrovoc/c_14037 http://aims.fao.org/aos/agrovoc/c_6259 |
spellingShingle |
L50 - Physiologie et biochimie animales Saumon Sodium Salmo salar Protéine http://aims.fao.org/aos/agrovoc/c_6756 http://aims.fao.org/aos/agrovoc/c_7145 http://aims.fao.org/aos/agrovoc/c_14037 http://aims.fao.org/aos/agrovoc/c_6259 D'Cotta, Helena Valotaire, Claudiane Le Gac, Florence Prunet, Patrick Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
topic_facet |
L50 - Physiologie et biochimie animales Saumon Sodium Salmo salar Protéine http://aims.fao.org/aos/agrovoc/c_6756 http://aims.fao.org/aos/agrovoc/c_7145 http://aims.fao.org/aos/agrovoc/c_14037 http://aims.fao.org/aos/agrovoc/c_6259 |
description |
Several parameters were analyzed to determine the mechanisms responsible for the enhancement of the gill Na+ -K+ -ATPase activity of Atlantic salmon smolts. A major alfa-subunit transcript of 3.7 kb was revealed by Northern blot in both parr and smolt gills when hybridized with two distinct cDNA probes. The alfa-mRNA abundance demonstrated an increase to maximal levels in smolts at an early stage of the parr-smolt transformation. This was followed by a gradual rise in alfa-protein levels, revealed by Western blots with specific antibodies and by an increase in gill Na+ -K+ -ATPase hydrolytic activity, both only reaching maximum levels a month later, at the peak of the transformation process. Parr fish experienced a decrease in alfa-mRNA abundance and had basal levels of alfa-protein and enzyme activity. Measurement of the binding of [3H]ouabain to Na+ -K+ -ATPase was characterized in smolts and parr gill membranes showing more than a twofold elevation in smolts and was of high affinity in both groups (dissociation constant = 20-23 nM). Modulation of the enzyme due to increased salinity was also observed in seawater-transferred smolts, as demonstrated by an increase in alfa-mRNA levels after 24 h with a rise in Na+ -K+ -ATPase activity occurring only after 11 days. No qualitative change in alfa-expression was revealed at either the mRNA or protein level. Immunological identification of the alfa-protein was performed with polyclonal antibodies directed against the rat alfa-specific isoforms, revealing that parr, freshwater, and seawater smolts have an alfa3-like isoform. This study shows that the increase in Na+ -K+ -ATPase activity in smolt gills depends first on an increase in the alfa-mRNA expression and is followed by a slower rise in -protein abundance that eventually leads to a higher synthesis of Na+ -K+ pumps. |
format |
Article in Journal/Newspaper |
author |
D'Cotta, Helena Valotaire, Claudiane Le Gac, Florence Prunet, Patrick |
author_facet |
D'Cotta, Helena Valotaire, Claudiane Le Gac, Florence Prunet, Patrick |
author_sort |
D'Cotta, Helena |
title |
Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
title_short |
Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
title_full |
Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
title_fullStr |
Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
title_full_unstemmed |
Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels |
title_sort |
synthesis of gill na+ -k+ -atpase in atlantic salmon smolts: differences in alfa-mrna and alfa-protein levels |
publishDate |
2000 |
url |
http://agritrop.cirad.fr/510591/ http://agritrop.cirad.fr/510591/1/510591.pdf https://doi.org/10.1152/ajpregu.2000.278.1.R101 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=175131 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology |
op_relation |
http://agritrop.cirad.fr/510591/ Synthesis of gill Na+ -K+ -ATPase in Atlantic salmon smolts: differences in alfa-mRNA and alfa-protein levels. D'Cotta Helena, Valotaire Claudiane, Le Gac Florence, Prunet Patrick. 2000. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 278 (1), R101-R110https://doi.org/10.1152/ajpregu.2000.278.1.R101 <https://doi.org/10.1152/ajpregu.2000.278.1.R101> http://agritrop.cirad.fr/510591/1/510591.pdf |
op_rights |
Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
op_doi |
https://doi.org/10.1152/ajpregu.2000.278.1.R101 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
278 |
container_issue |
1 |
container_start_page |
R101 |
op_container_end_page |
R110 |
_version_ |
1766362675753779200 |