Isolation and Characterization of Potential Probiotic Bacteria Suitable for Mollusk Larvae Cultures

Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoagg...

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Bibliographic Details
Published in:The Thai Journal of Veterinary Medicine
Main Authors: Escamilla-Montes, Ruth, Luna-González, Antonio, Flores-Miranda, Ma. del Carmen, Álvarez-Ruiz, Píndaro, Fierro-Coronado, Jesús Arturo, Sánchez-Ortiz, Ana Claudia, Ávila-Leal, José
Format: Text
Language:unknown
Published: Chula Digital Collections 2015
Subjects:
bacilli
lactic acid bacteria
larvae
mollusks
probiotics
Veterinary Medicine
Crassostrea gigas
Online Access:https://digital.car.chula.ac.th/tjvm/vol45/iss1/3
https://doi.org/10.56808/2985-1130.2614
https://digital.car.chula.ac.th/context/tjvm/article/2614/viewcontent/openpdf.php
Description
Summary:Guts of Crassostrea gigas, C. corteziensis, and Atrina maura were screened for bacilli and lactic acid bacteria with probiotic properties for bivalve mollusks. Characterization of the isolates included Gram stain, morphology, cell arrangement, catalase and hemolytic activity, hydrophobicity, autoaggregation, coaggregation against Vibrio sinaloensis VHPC23, biofilm formation, counting of colony forming units (CFU), NaCl and pH tolerance, enzymatic activity, resistance to antibiotics, antagonism against Vibrio sinaloensis VHPC23, and molecular identification. Three isolates (BCR 4-3, BALCR 5-2, and BALOR 2-3) showed no hemolytic activity, high tolerance to pH and salinity, high adhesion potential, high autoaggregation and coaggregation, biofilm formation, well growth, and some resistance to antibiotics. The isolates were identified as Bacillus licheniformis (BCR 4-3) and Leuconostoc mesenteroides (BALCR 5-2 and BALOR 2-3). The strains showed basic characteristics to be used as probiotics in mollusk larvae cultures.

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