Molecular cloning of grass carp (Ctenopharyngodon idellus) growth hormone gene.

• Other Authors: Wong, Mee-wa., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
• Format: Text
• Language: English
• Published: Chinese University of Hong Kong 1990
• Subjects: Molecular cloning; Ctenopharyngodon idella; Somatomedin; Lambda; Atlantic salmon
• Online Access: http://library.cuhk.edu.hk/record=b5886625; https://repository.lib.cuhk.edu.hk/en/item/cuhk-318558

by Wong Mee-wa. Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. Bibliography: leaves 100-104. Contents Abstract Abbreviations Chapter Chapter 1 --- General Introduction --- p.1 Chapter 1.1 --- Introduction --- p.1 Chapter 1.2 --- Biological Functions and Structure of GH --- p.1 Chapter 1.3 --- Molecular Cloning of GH cDNA and its Expression --- p.3 Chapter 1.4 --- Molecular Cloning of the Genomic Sequence of Growth Hormone --- p.5 Chapter 1.4.1 --- The Isolation of Mammalian GH gene --- p.5 Chapter 1.4.2 --- Molecular Cloning of Rainbow Trout Growth Hormone Gene --- p.6 Chapter 1.4.3 --- Molecular Cloning of Atlantic Salmon Growth Hormone Gene --- p.9 Chapter 1.4.4 --- Molecular Evolution of GH gene --- p.9 Chapter 1.4.5 --- Control Elements on GH gene --- p.12 Chapter 1.4.6 --- Production of Transgenic Fish --- p.14 Chapter 1.5 --- Purpose of present Study --- p.15 Chapter Chapter 2 --- Construction of a Genomic Library --- p.17 Chapter 2.1 --- Introduction --- p.17 Chapter 2.2 --- Strategy --- p.19 Chapter 2.3 --- Materials and Methods --- p.20 Chapter 2.3.1 --- Materials --- p.20 Chapter 2.3.2 --- Procedure --- p.24 Chapter 2.3.2.1 --- Extraction of Total Genomic DMA --- p.24 Chapter 2.3.2.2 --- Southern Blotting and Hybridization --- p.25 Chapter 2.3.2.3 --- Extraction of the GH Enriched DNA Fraction --- p.28 Chapter 2.3.2.4 --- Cloning into Phage Vector Lambda GT11 --- p.29 Chapter 2.3.2.5 --- Studies on the Enriched Genomic Library --- p.31 Chapter 2.4 --- Result --- p.33 Chapter 2.5 --- Discussion --- p.43 Chapter Chapter 3 --- Screening --- p.46 Chapter 3.1 --- Strategy --- p.46 Chapter 3.2 --- Materials and Methods --- p.47 Chapter 3.2.1 --- Materials --- p.47 Chapter 3.2.2 --- Procedure --- p.48 Chapter 3.2.2.1 --- Primary Screening --- p.48 Chapter 3.2.2.2 --- Purificaiton of Positive Signal --- p.49 Chapter 3.2.2.3 --- DNA Extraction from Positive Clone --- p.49 Chapter 3.3 --- Result --- p.50 Chapter 3.4 --- ...