Research and development for molecular authentication of food products

• Other Authors: But, Grace Wing Chiu (author.), Shaw, P.-C. (Pang-Chui) (thesis advisor.), Chinese University of Hong Kong Graduate School. Division of Life Sciences. (degree granting institution.)
• Format: Text
• Language: English; Chinese
• Published: 2019
• Subjects: Food > Analysis; Food adulteration and inspection; Food > Microbiology; Food Analysis > methods; Food Inspection; Food Microbiology; Molecular Biology; Cetorhinus maximus; Lamna nasus
• Online Access: https://julac.hosted.exlibrisgroup.com/primo-explore/search?query=addsrcrid,exact,991039842706503407,AND&tab=default_tab&search_scope=All&vid=CUHK&mode=advanced&lang=en_US; https://repository.lib.cuhk.edu.hk/en/item/cuhk-2327285

Ph.D. There is a growth of choosing organic food products and non endangered food sources. To protect both consumers and food sources and allow effective and efficient regulatory enforcement activities, there is a need to develop rapid molecular methods for instant on site identification. This study reports the development of molecular method for the identification of shark fin from endangered shark species and the identification of transgenic papaya using rapid nucleic acid amplficiation techniques. The translucent collagen fibers from shark fins are the primary ingredient of the Asian luxurious delicacy shark fin soup. This has driven worldwide overexploitation of sharks, threatening dozens of shark species. Effective from October 2017, twelve shark species, including Carcharhinus longimanus, Carcharhinus falciformis, Carcharodon carcharias, Cetorhinus maximus, Rhincodon typus, Lamna nasus, three species of hammerhead sharks (Sphyrna lewini, Sphyrna mokarran, Sphyrna zygaena), and three species of thresher sharks (Alopias pelagicus, Alopias superciliosus, Alopias vulpinus) are listed on Appendix II in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Shark samples in the form of dried fins, frozen tissues, and blood were collected from local and overseas. Samples collected include shark species commonly found in Asian marine territories and those closely related to the twelve endangered sharks. To allow effective law enforcement with species--level identification, species--specific assays targeting each of the CITES--listed shark species were developed based on the loop--mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) methods. Species--specific primers were designed based on cytochrome oxidase I (COI) and NADH dehydrogenase subunit 2 (NADH2) regions. Both LAMP and PCR assays developed were tested on 291 samples from 93 shark and related species. Target shark species were able to be differentiated from non--target species ...