A new primer-pair for sex identification of larks and wagtails
Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this...
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ftchinacascnwipb:oai:210.75.249.4:363003/27551 2023-05-15T16:06:20+02:00 A new primer-pair for sex identification of larks and wagtails Chu, Hui Guo, Xinyi Zeng, Yan Zou, Xiaoyan Guo, Songchang Zhao, Liang 2015-03-01 http://210.75.249.4/handle/363003/5577 http://210.75.249.4/handle/363003/17359 http://210.75.249.4/handle/363003/22455 http://210.75.249.4/handle/363003/27551 unknown CONSERVATION GENETICS RESOURCES http://210.75.249.4/handle/363003/5577 http://210.75.249.4/handle/363003/17359 http://210.75.249.4/handle/363003/22455 http://210.75.249.4/handle/363003/27551 null 期刊论文 2015 ftchinacascnwipb 2023-03-26T20:30:41Z Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity in birds. Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity ... Report Eremophila alpestris Northwest Institute of Plateau Biology: NWIPB OpenIR (Chinese Academy of Sciences) |
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Open Polar |
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Northwest Institute of Plateau Biology: NWIPB OpenIR (Chinese Academy of Sciences) |
op_collection_id |
ftchinacascnwipb |
language |
unknown |
description |
Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity in birds. Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity ... |
format |
Report |
author |
Chu, Hui Guo, Xinyi Zeng, Yan Zou, Xiaoyan Guo, Songchang Zhao, Liang |
spellingShingle |
Chu, Hui Guo, Xinyi Zeng, Yan Zou, Xiaoyan Guo, Songchang Zhao, Liang A new primer-pair for sex identification of larks and wagtails |
author_facet |
Chu, Hui Guo, Xinyi Zeng, Yan Zou, Xiaoyan Guo, Songchang Zhao, Liang |
author_sort |
Chu, Hui |
title |
A new primer-pair for sex identification of larks and wagtails |
title_short |
A new primer-pair for sex identification of larks and wagtails |
title_full |
A new primer-pair for sex identification of larks and wagtails |
title_fullStr |
A new primer-pair for sex identification of larks and wagtails |
title_full_unstemmed |
A new primer-pair for sex identification of larks and wagtails |
title_sort |
new primer-pair for sex identification of larks and wagtails |
publishDate |
2015 |
url |
http://210.75.249.4/handle/363003/5577 http://210.75.249.4/handle/363003/17359 http://210.75.249.4/handle/363003/22455 http://210.75.249.4/handle/363003/27551 |
genre |
Eremophila alpestris |
genre_facet |
Eremophila alpestris |
op_relation |
CONSERVATION GENETICS RESOURCES http://210.75.249.4/handle/363003/5577 http://210.75.249.4/handle/363003/17359 http://210.75.249.4/handle/363003/22455 http://210.75.249.4/handle/363003/27551 |
op_rights |
null |
_version_ |
1766402233421791232 |