RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China
Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Q...
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ftchinacascnwipb:oai:210.75.249.4:363003/1379 2023-05-15T18:15:15+02:00 RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China Chen, SL Xia, T Chen, SY Zhou, YJ 2005-04-01 http://ir.nwipb.ac.cn/handle/363003/1379 英语 eng BIOCHEMICAL GENETICS Chen Shilong, Xia Tao, Chen Shengyun, Zhou Yijun.RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China.BIOCHEMICAL GENETICS,2005,43(3-4): 189-201 http://ir.nwipb.ac.cn/handle/363003/1379 Coelonema Genetic Variation Rapd Qinghai-tibet Plateau 生物科学::生物化学 Science & Technology Life Sciences & Biomedicine SAXIFRAGA-OPPOSITIFOLIA POPULATIONS DIVERSITY MARKERS PLANT ALPS POLYMORPHISMS PHYLOGENY HISTORY CERNUA Biochemistry & Molecular Biology Genetics & Heredity Article 期刊论文 2005 ftchinacascnwipb 2023-03-26T20:22:06Z Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P. R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% ( 161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible. Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong ... Article in Journal/Newspaper Saxifraga oppositifolia Northwest Institute of Plateau Biology: NWIPB OpenIR (Chinese Academy of Sciences) |
institution |
Open Polar |
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Northwest Institute of Plateau Biology: NWIPB OpenIR (Chinese Academy of Sciences) |
op_collection_id |
ftchinacascnwipb |
language |
English |
topic |
Coelonema Genetic Variation Rapd Qinghai-tibet Plateau 生物科学::生物化学 Science & Technology Life Sciences & Biomedicine SAXIFRAGA-OPPOSITIFOLIA POPULATIONS DIVERSITY MARKERS PLANT ALPS POLYMORPHISMS PHYLOGENY HISTORY CERNUA Biochemistry & Molecular Biology Genetics & Heredity |
spellingShingle |
Coelonema Genetic Variation Rapd Qinghai-tibet Plateau 生物科学::生物化学 Science & Technology Life Sciences & Biomedicine SAXIFRAGA-OPPOSITIFOLIA POPULATIONS DIVERSITY MARKERS PLANT ALPS POLYMORPHISMS PHYLOGENY HISTORY CERNUA Biochemistry & Molecular Biology Genetics & Heredity Chen, SL Xia, T Chen, SY Zhou, YJ RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
topic_facet |
Coelonema Genetic Variation Rapd Qinghai-tibet Plateau 生物科学::生物化学 Science & Technology Life Sciences & Biomedicine SAXIFRAGA-OPPOSITIFOLIA POPULATIONS DIVERSITY MARKERS PLANT ALPS POLYMORPHISMS PHYLOGENY HISTORY CERNUA Biochemistry & Molecular Biology Genetics & Heredity |
description |
Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P. R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% ( 161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible. Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong ... |
format |
Article in Journal/Newspaper |
author |
Chen, SL Xia, T Chen, SY Zhou, YJ |
author_facet |
Chen, SL Xia, T Chen, SY Zhou, YJ |
author_sort |
Chen, SL |
title |
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
title_short |
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
title_full |
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
title_fullStr |
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
title_full_unstemmed |
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China |
title_sort |
rapd profiling in detecting genetic variation in endemic coelonema (brassicaceae) of qinghai-tibet plateau of china |
publishDate |
2005 |
url |
http://ir.nwipb.ac.cn/handle/363003/1379 |
genre |
Saxifraga oppositifolia |
genre_facet |
Saxifraga oppositifolia |
op_relation |
BIOCHEMICAL GENETICS Chen Shilong, Xia Tao, Chen Shengyun, Zhou Yijun.RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China.BIOCHEMICAL GENETICS,2005,43(3-4): 189-201 http://ir.nwipb.ac.cn/handle/363003/1379 |
_version_ |
1766188356412112896 |