A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas
Astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full le...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/95217 https://doi.org/10.1016/j.dci.2015.10.025 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/95217 2023-05-15T15:57:46+02:00 A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas Li, Yiqun Jiang, Shuai Li, Meijia Xin, Lusheng Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng 2016-02-01 http://ir.qdio.ac.cn/handle/337002/95217 https://doi.org/10.1016/j.dci.2015.10.025 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Yiqun,Jiang, Shuai,Li, Meijia,et al. A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):179-187. http://ir.qdio.ac.cn/handle/337002/95217 doi:10.1016/j.dci.2015.10.025 Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.dci.2015.10.025 2022-06-27T05:37:26Z Astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full length cDNA of CgAstakine encoded a polypeptide of 103 amino acids containing a prokineticin (PK) domain homologous to that in astakine from freshwater crayfish P. leniusculus. The deduced amino acid sequence of CgAstakine shared higher similarity with those of other invertebrate astakines than prokineticins from vertebrates. The mRNA of CgAstakine was highly expressed in hepatopancreas and adductor muscle of oyster, while the CgAstakine protein was mainly distributed in hepatopancreas, gill and hemocytes. The mRNA expression of CgAstaldne in hemocytes was significantly increased (p < 0.01) and maintained at a high level from 3 h to 9 h after Vibrio anguillarum challenge. After the oyster hemocytes were incubated with 5 mu g/mL recombinant CgAstakine protein (rCgAstakine) for 24 h in vitro, the proliferation of hemocytes was significantly increased to 1.89 fold of that in control group (p < 0.05). Moreover, the total count of oyster hemocytes was significantly upregulated (2.45 fold of that in control group, p < 0.05) at 12 h after the oysters were received an injection of rCgAstakine (0.5 mu g/g). These results collectively indicated that CgAstakine could modulate the hemocytes proliferation both in vitro and in vivo, and probably involved in the hematopoietic process fighting against the invasion of foreign pathogens. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 55 179 187 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation |
spellingShingle |
Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation Li, Yiqun Jiang, Shuai Li, Meijia Xin, Lusheng Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Hematopoiesis Innate Immunity Astakine Hemocyte Proliferation |
description |
Astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish Pacifastacus leniusculus. In the present study, an astakine homologous gene was identified from Pacific oyster Crassostrea gigas (designated CgAstakine). The full length cDNA of CgAstakine encoded a polypeptide of 103 amino acids containing a prokineticin (PK) domain homologous to that in astakine from freshwater crayfish P. leniusculus. The deduced amino acid sequence of CgAstakine shared higher similarity with those of other invertebrate astakines than prokineticins from vertebrates. The mRNA of CgAstakine was highly expressed in hepatopancreas and adductor muscle of oyster, while the CgAstakine protein was mainly distributed in hepatopancreas, gill and hemocytes. The mRNA expression of CgAstaldne in hemocytes was significantly increased (p < 0.01) and maintained at a high level from 3 h to 9 h after Vibrio anguillarum challenge. After the oyster hemocytes were incubated with 5 mu g/mL recombinant CgAstakine protein (rCgAstakine) for 24 h in vitro, the proliferation of hemocytes was significantly increased to 1.89 fold of that in control group (p < 0.05). Moreover, the total count of oyster hemocytes was significantly upregulated (2.45 fold of that in control group, p < 0.05) at 12 h after the oysters were received an injection of rCgAstakine (0.5 mu g/g). These results collectively indicated that CgAstakine could modulate the hemocytes proliferation both in vitro and in vivo, and probably involved in the hematopoietic process fighting against the invasion of foreign pathogens. (C) 2015 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Li, Yiqun Jiang, Shuai Li, Meijia Xin, Lusheng Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng |
author_facet |
Li, Yiqun Jiang, Shuai Li, Meijia Xin, Lusheng Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng |
author_sort |
Li, Yiqun |
title |
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
title_short |
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
title_full |
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
title_fullStr |
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
title_full_unstemmed |
A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas |
title_sort |
cytokine-like factor astakine accelerates the hemocyte production in pacific oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/95217 https://doi.org/10.1016/j.dci.2015.10.025 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Li, Yiqun,Jiang, Shuai,Li, Meijia,et al. A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):179-187. http://ir.qdio.ac.cn/handle/337002/95217 doi:10.1016/j.dci.2015.10.025 |
op_doi |
https://doi.org/10.1016/j.dci.2015.10.025 |
container_title |
Developmental & Comparative Immunology |
container_volume |
55 |
container_start_page |
179 |
op_container_end_page |
187 |
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1766393461396733952 |