An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials
The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rat...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/6148 2024-09-15T18:34:02+00:00 An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials Liu, Qinghua Li, Jun Zhang, Shicui Ding, Fuhong Xu, Xizhang Xiao, Zhizhong Xu, Shihong 2006-09-01 http://ir.qdio.ac.cn/handle/337002/6149 英语 eng JOURNAL OF THE WORLD AQUACULTURE SOCIETY Liu, Qinghua; Li, Jun; Zhang, Shicui; Ding, Fuhong; Xu, Xizhang; Xiao, Zhizhong; Xu, Shihong.An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials,JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2006,37(3):289-297 http://ir.qdio.ac.cn/handle/337002/6149 Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws Fisheries Article 期刊论文 2006 ftchinacasciocas 2024-08-09T03:18:11Z The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources. The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws Fisheries |
spellingShingle |
Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws Fisheries Liu, Qinghua Li, Jun Zhang, Shicui Ding, Fuhong Xu, Xizhang Xiao, Zhizhong Xu, Shihong An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
topic_facet |
Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws Fisheries |
description |
The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources. The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling ... |
format |
Article in Journal/Newspaper |
author |
Liu, Qinghua Li, Jun Zhang, Shicui Ding, Fuhong Xu, Xizhang Xiao, Zhizhong Xu, Shihong |
author_facet |
Liu, Qinghua Li, Jun Zhang, Shicui Ding, Fuhong Xu, Xizhang Xiao, Zhizhong Xu, Shihong |
author_sort |
Liu, Qinghua |
title |
An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
title_short |
An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
title_full |
An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
title_fullStr |
An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
title_full_unstemmed |
An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials |
title_sort |
efficient methodology for cryopreservation of spermatozoa of red seabream, pagrus major, with 2-ml cryovials |
publishDate |
2006 |
url |
http://ir.qdio.ac.cn/handle/337002/6149 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
JOURNAL OF THE WORLD AQUACULTURE SOCIETY Liu, Qinghua; Li, Jun; Zhang, Shicui; Ding, Fuhong; Xu, Xizhang; Xiao, Zhizhong; Xu, Shihong.An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials,JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2006,37(3):289-297 http://ir.qdio.ac.cn/handle/337002/6149 |
_version_ |
1810475756462014464 |