Construction and analysis of an experimental Streptococcus iniae DNA vaccine
Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examine...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/5924 2024-09-15T18:40:00+00:00 Construction and analysis of an experimental Streptococcus iniae DNA vaccine Sun, Yun Hu, Yong-hua Liu, Chun-sheng Sun, Li 2010-05-21 http://ir.qdio.ac.cn/handle/337002/5925 https://doi.org/10.1016/j.vaccine.2010.03.071 英语 eng VACCINE Sun, Yun; Hu, Yong-hua; Liu, Chun-sheng; Sun, Li.Construction and analysis of an experimental Streptococcus iniae DNA vaccine,VACCINE,2010,28(23):3905-3912 http://ir.qdio.ac.cn/handle/337002/5925 doi:10.1016/j.vaccine.2010.03.071 Streptococcus Iniae Dna Vaccine Immune Genes Passive Immunization Immunology Medicine Research & Experimental Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1016/j.vaccine.2010.03.071 2024-08-09T03:18:11Z Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 under the cytomegalovirus immediate-early promoter. In fish vaccinated with pSia10, transcription of sia10 was detected in muscle, liver, spleen, and kidney at 7, 14, 21, 28, 35, 42, and 49 days post-vaccination. In addition, production of Sia10 protein was also detected in the muscle tissues of pSia10-vaccinated fish. Fish vaccinated with pSia10 exhibited a relative percent survival (RPS) of 73.9% and 92.3%, respectively, when challenged with high and low doses (producing a cumulative mortality of 92% and 52%, respectively, in the control groups) of S. iniae. Immunological and transcriptional analyses showed that vaccination with pSia10(i) induced much stronger chemiluminescence response and significantly higher levels of nitric oxide production and acid phosphatase activity in head kidney macrophages; (ii) caused the production of specific serum antibodies, which afforded apparent immunoprotection when transferred passively into naive fish; and (iii) upregulated the expression of the genes encoding proteins that are possibly involved in both innate and adaptive immune responses. Taken together, these results indicated that pSia10 is an effective vaccine candidate and may be used in the control of S. iniae infection in aquaculture. (C) 2010 Elsevier Ltd. All rights reserved. Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 ... Article in Journal/Newspaper Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Vaccine 28 23 3905 3912 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Streptococcus Iniae Dna Vaccine Immune Genes Passive Immunization Immunology Medicine Research & Experimental |
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Streptococcus Iniae Dna Vaccine Immune Genes Passive Immunization Immunology Medicine Research & Experimental Sun, Yun Hu, Yong-hua Liu, Chun-sheng Sun, Li Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
topic_facet |
Streptococcus Iniae Dna Vaccine Immune Genes Passive Immunization Immunology Medicine Research & Experimental |
description |
Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 under the cytomegalovirus immediate-early promoter. In fish vaccinated with pSia10, transcription of sia10 was detected in muscle, liver, spleen, and kidney at 7, 14, 21, 28, 35, 42, and 49 days post-vaccination. In addition, production of Sia10 protein was also detected in the muscle tissues of pSia10-vaccinated fish. Fish vaccinated with pSia10 exhibited a relative percent survival (RPS) of 73.9% and 92.3%, respectively, when challenged with high and low doses (producing a cumulative mortality of 92% and 52%, respectively, in the control groups) of S. iniae. Immunological and transcriptional analyses showed that vaccination with pSia10(i) induced much stronger chemiluminescence response and significantly higher levels of nitric oxide production and acid phosphatase activity in head kidney macrophages; (ii) caused the production of specific serum antibodies, which afforded apparent immunoprotection when transferred passively into naive fish; and (iii) upregulated the expression of the genes encoding proteins that are possibly involved in both innate and adaptive immune responses. Taken together, these results indicated that pSia10 is an effective vaccine candidate and may be used in the control of S. iniae infection in aquaculture. (C) 2010 Elsevier Ltd. All rights reserved. Streptococcus iniae is a severe aquaculture pathogen that can also infect humans and animal. A putative secretory antigen, Slat 0, was identified from a pathogenic S. iniae strain by in vivo-induced antigen technology. Using turbot as an animal model, the immunoprotective effect of Sia10 was examined as a DNA vaccine in the form of plasmid pSia10, which expresses sia10 ... |
format |
Article in Journal/Newspaper |
author |
Sun, Yun Hu, Yong-hua Liu, Chun-sheng Sun, Li |
author_facet |
Sun, Yun Hu, Yong-hua Liu, Chun-sheng Sun, Li |
author_sort |
Sun, Yun |
title |
Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
title_short |
Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
title_full |
Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
title_fullStr |
Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
title_full_unstemmed |
Construction and analysis of an experimental Streptococcus iniae DNA vaccine |
title_sort |
construction and analysis of an experimental streptococcus iniae dna vaccine |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/5925 https://doi.org/10.1016/j.vaccine.2010.03.071 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
VACCINE Sun, Yun; Hu, Yong-hua; Liu, Chun-sheng; Sun, Li.Construction and analysis of an experimental Streptococcus iniae DNA vaccine,VACCINE,2010,28(23):3905-3912 http://ir.qdio.ac.cn/handle/337002/5925 doi:10.1016/j.vaccine.2010.03.071 |
op_doi |
https://doi.org/10.1016/j.vaccine.2010.03.071 |
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Vaccine |
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28 |
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23 |
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3905 |
op_container_end_page |
3912 |
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1810484329664479232 |