Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection
ISG15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. ISG15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysacchande (LPS) treatment However, the relationship between ISG1...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/5872 2024-09-15T18:32:13+00:00 Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection Liu, Chun-sheng Sun, Yun Zhang, Min Sun, Li 2010-07-01 http://ir.qdio.ac.cn/handle/337002/5873 https://doi.org/10.1016/j.fsi.2010.03.012 英语 eng FISH & SHELLFISH IMMUNOLOGY Liu, Chunsheng; Sun, Yun; Zhang, Min; Sun, Li.Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection,FISH & SHELLFISH IMMUNOLOGY,2010,29(1):167-174 http://ir.qdio.ac.cn/handle/337002/5873 doi:10.1016/j.fsi.2010.03.012 Isg15 Sciaenops Ocellatus Macrophage Activation Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1016/j.fsi.2010.03.012 2024-08-09T03:18:11Z ISG15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. ISG15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysacchande (LPS) treatment However, the relationship between ISG15 and live bacterial infection has not been investigated in piscine models. In this study, an ISG15 homologue, SoISG15, was identified from red drum Scraeriops ocellaws and analyzed at expression and functional levels The open reading frame ofSolSG15 is 477 base pairs (bp) and mtronless, with a 5'-untranslated region (UTR) of 91 bp and a 3'-UTR of 415 bp The deduced amino acid sequence of S0ISG15 shares 60-67% overall identities with the ISG15 of several fish species. S0ISG15 possesses two conserved ubiquinn-like domains and the canonical ubiquitin conjugation motif, LRGG, at the C-terminus. Expressional analysis showed that constitutive expression of SolSG15 was highest in blood and lowest in kidney Experimental challenges with LPS and bacterial pathogens induced significant S0ISG15 expression in the kidney but not in the liver Similar differential induction was also observed at cellular level with primary hepatocytes and head kidney (HK) lymphocytes. Poly(' C), however, effected drastic induction of S0ISG15 expression in kidney and liver at both tissue and cellular levels. Immunoblot analysis showed that S0ISG15 was secreted by cultured HK lymphocytes into the extracellular milieu. Recombinant S0ISG15 expressed in and purified from Eschenclua colt was able to enhance the respiratory burst activity, acid phosphatase activity, and bactericidal activity of HK macrophages. Taken together, the results of this study indicated that SoISG 15 possesses apparent immunological property and is likely to be involved in host immune defense against bacterial infection. (C)2010 Elsevier Ltd All rights reserved. ISG15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. ISG15 homologues have been identified in a ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 29 1 167 174 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Isg15 Sciaenops Ocellatus Macrophage Activation Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Isg15 Sciaenops Ocellatus Macrophage Activation Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Liu, Chun-sheng Sun, Yun Zhang, Min Sun, Li Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
topic_facet |
Isg15 Sciaenops Ocellatus Macrophage Activation Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
ISG15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. ISG15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysacchande (LPS) treatment However, the relationship between ISG15 and live bacterial infection has not been investigated in piscine models. In this study, an ISG15 homologue, SoISG15, was identified from red drum Scraeriops ocellaws and analyzed at expression and functional levels The open reading frame ofSolSG15 is 477 base pairs (bp) and mtronless, with a 5'-untranslated region (UTR) of 91 bp and a 3'-UTR of 415 bp The deduced amino acid sequence of S0ISG15 shares 60-67% overall identities with the ISG15 of several fish species. S0ISG15 possesses two conserved ubiquinn-like domains and the canonical ubiquitin conjugation motif, LRGG, at the C-terminus. Expressional analysis showed that constitutive expression of SolSG15 was highest in blood and lowest in kidney Experimental challenges with LPS and bacterial pathogens induced significant S0ISG15 expression in the kidney but not in the liver Similar differential induction was also observed at cellular level with primary hepatocytes and head kidney (HK) lymphocytes. Poly(' C), however, effected drastic induction of S0ISG15 expression in kidney and liver at both tissue and cellular levels. Immunoblot analysis showed that S0ISG15 was secreted by cultured HK lymphocytes into the extracellular milieu. Recombinant S0ISG15 expressed in and purified from Eschenclua colt was able to enhance the respiratory burst activity, acid phosphatase activity, and bactericidal activity of HK macrophages. Taken together, the results of this study indicated that SoISG 15 possesses apparent immunological property and is likely to be involved in host immune defense against bacterial infection. (C)2010 Elsevier Ltd All rights reserved. ISG15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. ISG15 homologues have been identified in a ... |
format |
Article in Journal/Newspaper |
author |
Liu, Chun-sheng Sun, Yun Zhang, Min Sun, Li |
author_facet |
Liu, Chun-sheng Sun, Yun Zhang, Min Sun, Li |
author_sort |
Liu, Chun-sheng |
title |
Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
title_short |
Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
title_full |
Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
title_fullStr |
Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
title_full_unstemmed |
Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection |
title_sort |
identification and analysis of a sciaenops ocellatus isg15 homologue that is involved in host immune defense against bacterial infection |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/5873 https://doi.org/10.1016/j.fsi.2010.03.012 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Liu, Chunsheng; Sun, Yun; Zhang, Min; Sun, Li.Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection,FISH & SHELLFISH IMMUNOLOGY,2010,29(1):167-174 http://ir.qdio.ac.cn/handle/337002/5873 doi:10.1016/j.fsi.2010.03.012 |
op_doi |
https://doi.org/10.1016/j.fsi.2010.03.012 |
container_title |
Fish & Shellfish Immunology |
container_volume |
29 |
container_issue |
1 |
container_start_page |
167 |
op_container_end_page |
174 |
_version_ |
1810473969644470272 |