Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity
Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs in certain contexts are known to be immunostimulatory in vertebrate systems. CpG ODNs with immune effects have been identified for many fish species but, to our knowledge, not for turbot. In this study, a turbot-effective CpG ODN, ODN 2...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/5870 2024-09-15T18:34:00+00:00 Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity Liu, Chun-Sheng Sun, Yun Hu, Yong-hua Sun, Li 2010-06-07 http://ir.qdio.ac.cn/handle/337002/5871 https://doi.org/10.1016/j.vaccine.2010.04.016 英语 eng VACCINE Liu, ChunSheng; Sun, Yun; Hu, Yonghua; Sun, Li.Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity,VACCINE,2010,28(25):4153-4161 http://ir.qdio.ac.cn/handle/337002/5871 doi:10.1016/j.vaccine.2010.04.016 Cpg Odn Adjuvant Scophthalmus Maximus Immunostimulant Immunology Medicine Research & Experimental Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1016/j.vaccine.2010.04.016 2024-08-09T03:18:11Z Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs in certain contexts are known to be immunostimulatory in vertebrate systems. CpG ODNs with immune effects have been identified for many fish species but, to our knowledge, not for turbot. In this study, a turbot-effective CpG ODN, ODN 205, was identified and a plasmid, pCN5, was constructed which contains the CpG motif of ODN 205. When administered into turbot via intraperitoneal (i.p.) injection, both ODN 205 and pCN5 could (i) inhibit bacterial dissemination in blood in dose and time dependent manners, and (ii) protect against lethal bacterial challenge. Immunological analyses showed that in vitro treatment with ODN 205 stimulated peripheral blood leukocyte proliferation, while i.p. injection with ODN 205 enhanced the respiratory burst activity, chemiluminescence response, and acid phosphatase activity of turbot head kidney macrophages. pCN5 treatment-induced immune responses similar to those induced by ODN 205 treatment except that pCN5 could also enhance serum bactericidal activity in a calcium-independent manner. To examine whether ODN 205 and pCN5 had any effect on specific immunity, ODN 205 and pCN5 were co-administered into turbot with a Vibrio harveyi subunit vaccine, DegQ. The results showed that pCN5, but not ODN 205, significantly increased the immunoprotective efficacy of DegQ and enhanced the production of specific serum antibodies in the vaccinated fish. Further analysis indicated that vaccination with DegQ in the presence of pCN5 upregulated the expression of the genes encoding MHC class II alpha, IgM, Mx, and IL-8 receptor. Taken together, these results demonstrate that ODN 205 and pCN5 can stimulate the immune system of turbot and induce protection against bacterial challenge. In addition, pCN5 also possesses adjuvant property and can potentiate vaccine-induced specific immunity. (C) 2010 Elsevier Ltd. All rights reserved. Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs in certain contexts are known to be ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Vaccine 28 25 4153 4161 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Cpg Odn Adjuvant Scophthalmus Maximus Immunostimulant Immunology Medicine Research & Experimental |
spellingShingle |
Cpg Odn Adjuvant Scophthalmus Maximus Immunostimulant Immunology Medicine Research & Experimental Liu, Chun-Sheng Sun, Yun Hu, Yong-hua Sun, Li Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
topic_facet |
Cpg Odn Adjuvant Scophthalmus Maximus Immunostimulant Immunology Medicine Research & Experimental |
description |
Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs in certain contexts are known to be immunostimulatory in vertebrate systems. CpG ODNs with immune effects have been identified for many fish species but, to our knowledge, not for turbot. In this study, a turbot-effective CpG ODN, ODN 205, was identified and a plasmid, pCN5, was constructed which contains the CpG motif of ODN 205. When administered into turbot via intraperitoneal (i.p.) injection, both ODN 205 and pCN5 could (i) inhibit bacterial dissemination in blood in dose and time dependent manners, and (ii) protect against lethal bacterial challenge. Immunological analyses showed that in vitro treatment with ODN 205 stimulated peripheral blood leukocyte proliferation, while i.p. injection with ODN 205 enhanced the respiratory burst activity, chemiluminescence response, and acid phosphatase activity of turbot head kidney macrophages. pCN5 treatment-induced immune responses similar to those induced by ODN 205 treatment except that pCN5 could also enhance serum bactericidal activity in a calcium-independent manner. To examine whether ODN 205 and pCN5 had any effect on specific immunity, ODN 205 and pCN5 were co-administered into turbot with a Vibrio harveyi subunit vaccine, DegQ. The results showed that pCN5, but not ODN 205, significantly increased the immunoprotective efficacy of DegQ and enhanced the production of specific serum antibodies in the vaccinated fish. Further analysis indicated that vaccination with DegQ in the presence of pCN5 upregulated the expression of the genes encoding MHC class II alpha, IgM, Mx, and IL-8 receptor. Taken together, these results demonstrate that ODN 205 and pCN5 can stimulate the immune system of turbot and induce protection against bacterial challenge. In addition, pCN5 also possesses adjuvant property and can potentiate vaccine-induced specific immunity. (C) 2010 Elsevier Ltd. All rights reserved. Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs in certain contexts are known to be ... |
format |
Article in Journal/Newspaper |
author |
Liu, Chun-Sheng Sun, Yun Hu, Yong-hua Sun, Li |
author_facet |
Liu, Chun-Sheng Sun, Yun Hu, Yong-hua Sun, Li |
author_sort |
Liu, Chun-Sheng |
title |
Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
title_short |
Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
title_full |
Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
title_fullStr |
Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
title_full_unstemmed |
Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
title_sort |
identification and analysis of a cpg motif that protects turbot (scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/5871 https://doi.org/10.1016/j.vaccine.2010.04.016 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
VACCINE Liu, ChunSheng; Sun, Yun; Hu, Yonghua; Sun, Li.Identification and analysis of a CpG motif that protects turbot (Scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity,VACCINE,2010,28(25):4153-4161 http://ir.qdio.ac.cn/handle/337002/5871 doi:10.1016/j.vaccine.2010.04.016 |
op_doi |
https://doi.org/10.1016/j.vaccine.2010.04.016 |
container_title |
Vaccine |
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28 |
container_issue |
25 |
container_start_page |
4153 |
op_container_end_page |
4161 |
_version_ |
1810475734932652032 |