Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus
Hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In this study, an Hsp70 homologue (SoHsp70) was identified from red drum Sciaenops ocellatus and analyzed at molecular level. The open reading frame of SoHsp70 is 1920 bp and intronless, wit...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/5844 2023-05-15T18:06:03+02:00 Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus Dang, Wei Hu, Yong-hua Zhang, Min Sun, Li 2010-10-01 http://ir.qdio.ac.cn/handle/337002/5845 https://doi.org/10.1016/j.fsi.2010.06.001 英语 eng FISH & SHELLFISH IMMUNOLOGY Dang, Wei; Hu, Yong-hua; Zhang, Min; Sun, Li.Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus,FISH & SHELLFISH IMMUNOLOGY,2010,29(4):600-607 http://ir.qdio.ac.cn/handle/337002/5845 doi:10.1016/j.fsi.2010.06.001 Heat Shock Protein Hsp70 Sciaenops Ocellatus Stress Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1016/j.fsi.2010.06.001 2022-06-27T05:32:49Z Hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In this study, an Hsp70 homologue (SoHsp70) was identified from red drum Sciaenops ocellatus and analyzed at molecular level. The open reading frame of SoHsp70 is 1920 bp and intronless, with a 5'-untranslated region (UTR) of 399 bp and a 3'-UTR of 241 bp. The deduced amino acid sequence of SoHsp70 shares 84-92% overall identities with the Hsp70s of a number of fish species. In silico analysis identified in SoHsp70 three conserved Hsp70 domains involved in nucleotide and substrate binding. The coding sequence of SoHsp70 was subcloned into Escherichia coli, from which recombinant SoHsp70 was purified and, upon ATPase assay, found to exhibit apparent ATPase activity. Expressional analysis showed that constitutive expression of SoHsp70 was detectable in heart, liver, spleen, kidney, brain, blood, and gill. Experimental challenges with poly(I:C) and bacterial pathogens of Gram-positive and Gram-negative nature induced SoHsp70 expression in kidney to different levels. Stress-responsive analysis of SoHsp70 expression in primary cultures of red drum hepatocytes showed that acute heat shock treatment elicited a rapid induction of SoHsp70 expression which appeared after 10 min and 30 min of treatment. Exposure of hepatocytes separately to iron, copper, mercury, and hydrogen peroxide significantly unregulated SoHsp70 expression in time-dependent manners. Vaccination of red drum with a Streptococcus iniae bacterin was also found to induce SoHsp70 expression. Furthermore, recombinant SoHsp70 enhanced the immunoprotective effect of a subunit vaccine. Taken together, these results suggest that SoHsp70 is a stress-inducible protein that is likely to play a role in immunity and in coping with environmental and biological stresses. (C) 2010 Elsevier Ltd. All rights reserved. Hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In this study, an Hsp70 homologue ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 29 4 600 607 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Heat Shock Protein Hsp70 Sciaenops Ocellatus Stress Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Heat Shock Protein Hsp70 Sciaenops Ocellatus Stress Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Dang, Wei Hu, Yong-hua Zhang, Min Sun, Li Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
topic_facet |
Heat Shock Protein Hsp70 Sciaenops Ocellatus Stress Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In this study, an Hsp70 homologue (SoHsp70) was identified from red drum Sciaenops ocellatus and analyzed at molecular level. The open reading frame of SoHsp70 is 1920 bp and intronless, with a 5'-untranslated region (UTR) of 399 bp and a 3'-UTR of 241 bp. The deduced amino acid sequence of SoHsp70 shares 84-92% overall identities with the Hsp70s of a number of fish species. In silico analysis identified in SoHsp70 three conserved Hsp70 domains involved in nucleotide and substrate binding. The coding sequence of SoHsp70 was subcloned into Escherichia coli, from which recombinant SoHsp70 was purified and, upon ATPase assay, found to exhibit apparent ATPase activity. Expressional analysis showed that constitutive expression of SoHsp70 was detectable in heart, liver, spleen, kidney, brain, blood, and gill. Experimental challenges with poly(I:C) and bacterial pathogens of Gram-positive and Gram-negative nature induced SoHsp70 expression in kidney to different levels. Stress-responsive analysis of SoHsp70 expression in primary cultures of red drum hepatocytes showed that acute heat shock treatment elicited a rapid induction of SoHsp70 expression which appeared after 10 min and 30 min of treatment. Exposure of hepatocytes separately to iron, copper, mercury, and hydrogen peroxide significantly unregulated SoHsp70 expression in time-dependent manners. Vaccination of red drum with a Streptococcus iniae bacterin was also found to induce SoHsp70 expression. Furthermore, recombinant SoHsp70 enhanced the immunoprotective effect of a subunit vaccine. Taken together, these results suggest that SoHsp70 is a stress-inducible protein that is likely to play a role in immunity and in coping with environmental and biological stresses. (C) 2010 Elsevier Ltd. All rights reserved. Hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In this study, an Hsp70 homologue ... |
format |
Article in Journal/Newspaper |
author |
Dang, Wei Hu, Yong-hua Zhang, Min Sun, Li |
author_facet |
Dang, Wei Hu, Yong-hua Zhang, Min Sun, Li |
author_sort |
Dang, Wei |
title |
Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
title_short |
Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
title_full |
Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
title_fullStr |
Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
title_full_unstemmed |
Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus |
title_sort |
identification and molecular analysis of a stress-inducible hsp70 from sciaenops ocellatus |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/5845 https://doi.org/10.1016/j.fsi.2010.06.001 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Dang, Wei; Hu, Yong-hua; Zhang, Min; Sun, Li.Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus,FISH & SHELLFISH IMMUNOLOGY,2010,29(4):600-607 http://ir.qdio.ac.cn/handle/337002/5845 doi:10.1016/j.fsi.2010.06.001 |
op_doi |
https://doi.org/10.1016/j.fsi.2010.06.001 |
container_title |
Fish & Shellfish Immunology |
container_volume |
29 |
container_issue |
4 |
container_start_page |
600 |
op_container_end_page |
607 |
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1766177632952516608 |