Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus
Peroxiredoxins (Prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. To date, six different isoforms of peroxiredoxin (Prx1 to Prx6) have been identified, of which, Prx6 belongs to the 1-Cys Prx subfamily. Although Prx6 of several fish speci...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/5790 2024-09-15T18:34:00+00:00 Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus Zheng, Wen-jiang Hu, Yong-hua Zhang, Min Sun, Li 2010-08-01 http://ir.qdio.ac.cn/handle/337002/5791 https://doi.org/10.1016/j.fsi.2010.04.008 英语 eng FISH & SHELLFISH IMMUNOLOGY Zheng, Wen-jiang; Hu, Yong-hua; Zhang, Min; Sun, Li.Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus,FISH & SHELLFISH IMMUNOLOGY,2010,29(2):305-311 http://ir.qdio.ac.cn/handle/337002/5791 doi:10.1016/j.fsi.2010.04.008 Peroxiredoxin Scophthalmus Maximus Antioxidant Oxidative Stress Reactive Oxygen Species Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1016/j.fsi.2010.04.008 2024-08-09T03:18:11Z Peroxiredoxins (Prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. To date, six different isoforms of peroxiredoxin (Prx1 to Prx6) have been identified, of which, Prx6 belongs to the 1-Cys Prx subfamily. Although Prx6 of several fish species have been reported at sequence level, there are very few documented studies on the potential function of fish Prx6. In this report, we describe the identification and analysis of a Prx6 homologue, SmPrx6, from turbot Scophthalmus maximus. The full length cDNA of SmPrx6 contains a 5'- untranslated region (UTR) of 60 bp, an open reading frame of 666 bp, and a 3'-UTR of 244 bp. The deduced amino acid sequence of SmPrx6 shares 81-87% overall identities with known fish Prx6. In silico analysis identified in SmPrx6 a conserved Prx6 catalytic motif, PVCTTE, and the catalytic triads putatively involved in peroxidase and phospholipase A2 activities. Expression of SmPrx6 was detected in most fish organs, with the highest expression levels found in blood and heart and the lowest level in spleen. Experimental challenges with bacterial pathogens and poly(I:C) upregulated SmPrx6 expression in liver and spleen in a manner that is dependent on the challenging agent and the tissue type. Treatment of cultured primary hepatocytes with H(2)O(2) enhanced SmPrx6 expression in a dose-dependent manner. Recombinant SmPrx6 expressed in and purified from Escherichia coli exhibited thiol-dependent antioxidant activity and could protect cultured hepatocytes from H(2)O(2)-induced oxidative damage. Taken together, these results indicate that SmPrx6 is a Prx6 homologue with antioxidative property and is likely to be involved in both cellular maintenance and protective response during host immune defense against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. Peroxiredoxins (Prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. To date, six different isoforms of ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 29 2 305 311 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Peroxiredoxin Scophthalmus Maximus Antioxidant Oxidative Stress Reactive Oxygen Species Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Peroxiredoxin Scophthalmus Maximus Antioxidant Oxidative Stress Reactive Oxygen Species Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Zheng, Wen-jiang Hu, Yong-hua Zhang, Min Sun, Li Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
topic_facet |
Peroxiredoxin Scophthalmus Maximus Antioxidant Oxidative Stress Reactive Oxygen Species Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Peroxiredoxins (Prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. To date, six different isoforms of peroxiredoxin (Prx1 to Prx6) have been identified, of which, Prx6 belongs to the 1-Cys Prx subfamily. Although Prx6 of several fish species have been reported at sequence level, there are very few documented studies on the potential function of fish Prx6. In this report, we describe the identification and analysis of a Prx6 homologue, SmPrx6, from turbot Scophthalmus maximus. The full length cDNA of SmPrx6 contains a 5'- untranslated region (UTR) of 60 bp, an open reading frame of 666 bp, and a 3'-UTR of 244 bp. The deduced amino acid sequence of SmPrx6 shares 81-87% overall identities with known fish Prx6. In silico analysis identified in SmPrx6 a conserved Prx6 catalytic motif, PVCTTE, and the catalytic triads putatively involved in peroxidase and phospholipase A2 activities. Expression of SmPrx6 was detected in most fish organs, with the highest expression levels found in blood and heart and the lowest level in spleen. Experimental challenges with bacterial pathogens and poly(I:C) upregulated SmPrx6 expression in liver and spleen in a manner that is dependent on the challenging agent and the tissue type. Treatment of cultured primary hepatocytes with H(2)O(2) enhanced SmPrx6 expression in a dose-dependent manner. Recombinant SmPrx6 expressed in and purified from Escherichia coli exhibited thiol-dependent antioxidant activity and could protect cultured hepatocytes from H(2)O(2)-induced oxidative damage. Taken together, these results indicate that SmPrx6 is a Prx6 homologue with antioxidative property and is likely to be involved in both cellular maintenance and protective response during host immune defense against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. Peroxiredoxins (Prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. To date, six different isoforms of ... |
format |
Article in Journal/Newspaper |
author |
Zheng, Wen-jiang Hu, Yong-hua Zhang, Min Sun, Li |
author_facet |
Zheng, Wen-jiang Hu, Yong-hua Zhang, Min Sun, Li |
author_sort |
Zheng, Wen-jiang |
title |
Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
title_short |
Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
title_full |
Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
title_fullStr |
Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
title_full_unstemmed |
Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus |
title_sort |
analysis of the expression and antioxidative property of a peroxiredoxin 6 from scophthalmus maximus |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/5791 https://doi.org/10.1016/j.fsi.2010.04.008 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Zheng, Wen-jiang; Hu, Yong-hua; Zhang, Min; Sun, Li.Analysis of the expression and antioxidative property of a peroxiredoxin 6 from Scophthalmus maximus,FISH & SHELLFISH IMMUNOLOGY,2010,29(2):305-311 http://ir.qdio.ac.cn/handle/337002/5791 doi:10.1016/j.fsi.2010.04.008 |
op_doi |
https://doi.org/10.1016/j.fsi.2010.04.008 |
container_title |
Fish & Shellfish Immunology |
container_volume |
29 |
container_issue |
2 |
container_start_page |
305 |
op_container_end_page |
311 |
_version_ |
1810475725233324032 |