CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules
Interleulcin 17 (IL17) is a proinflammatory cytokine that plays an important role in immune response. Recently, five novel IL17 homologs have been identified by screening and analyzing the genome of pacific oyster Crassostrea gigas. In the present study, the functions of CgIL17-5 were investigated b...
Published in: | Developmental & Comparative Immunology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/51033 https://doi.org/10.1016/j.dci.2015.08.002 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/51033 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/51033 2023-05-15T15:57:46+02:00 CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules Xin, Lusheng Zhang, Huan Zhang, Ran Li, Hui Wang, Weilin Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng 2015-12-01 http://ir.qdio.ac.cn/handle/337002/51033 https://doi.org/10.1016/j.dci.2015.08.002 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Xin, Lusheng,Zhang, Huan,Zhang, Ran,et al. CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(2):339-348. http://ir.qdio.ac.cn/handle/337002/51033 doi:10.1016/j.dci.2015.08.002 Crassostrea Gigas Interleukin 17 Inflammation Pathogen-associated Molecular Pattern Recognition Bacteriostasis Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.dci.2015.08.002 2022-06-27T05:37:20Z Interleulcin 17 (IL17) is a proinflammatory cytokine that plays an important role in immune response. Recently, five novel IL17 homologs have been identified by screening and analyzing the genome of pacific oyster Crassostrea gigas. In the present study, the functions of CgIL17-5 were investigated by examining the distribution of its mRNA and protein, ligands binding and modulation in immune response. The mRNA expression levels of CgIL17-5 in hemocytes of oysters post twice challenges of Vibrio splendidus were all significantly up-regulated (P < 0.01), while the secondary pathogen infection attenuated the expression level of CgIL17-5 mRNA compared with the primary challenge. CgIL17-5 was found to be located on oyster hemocyte membranes through fluorescence confocal assay. The luciferase reporter assays showed that CgIL17-5 could activate the transfactors NF-kappa B, CREB and ATF-1, and involve in their signal pathways in HEK293T cells. Meanwhile, CgIL17-5 could augment the IL6 synthesis in HuVEC cells, playing the similar roles as human IL17 in inflammatory response. Additionally, the recombinant CgIL175 (rCgIL17-5) could directly bind peptidoglycan (PGN), lipopolysaccharide (LPS), poly (I:C) and beta-1,3-glucan, with the highest affinity to PGN, and significantly inhibit the growth of Micrococcus luteus and Escherichia coli. All the results collectively suggested that CgIL17-5, as an ancient inflammatory cytokine, could not only activate signal transduction for the release of other cytokines, but also mediate the clearance of extracellular bacteria in oysters. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 53 2 339 348 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Interleukin 17 Inflammation Pathogen-associated Molecular Pattern Recognition Bacteriostasis |
spellingShingle |
Crassostrea Gigas Interleukin 17 Inflammation Pathogen-associated Molecular Pattern Recognition Bacteriostasis Xin, Lusheng Zhang, Huan Zhang, Ran Li, Hui Wang, Weilin Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
topic_facet |
Crassostrea Gigas Interleukin 17 Inflammation Pathogen-associated Molecular Pattern Recognition Bacteriostasis |
description |
Interleulcin 17 (IL17) is a proinflammatory cytokine that plays an important role in immune response. Recently, five novel IL17 homologs have been identified by screening and analyzing the genome of pacific oyster Crassostrea gigas. In the present study, the functions of CgIL17-5 were investigated by examining the distribution of its mRNA and protein, ligands binding and modulation in immune response. The mRNA expression levels of CgIL17-5 in hemocytes of oysters post twice challenges of Vibrio splendidus were all significantly up-regulated (P < 0.01), while the secondary pathogen infection attenuated the expression level of CgIL17-5 mRNA compared with the primary challenge. CgIL17-5 was found to be located on oyster hemocyte membranes through fluorescence confocal assay. The luciferase reporter assays showed that CgIL17-5 could activate the transfactors NF-kappa B, CREB and ATF-1, and involve in their signal pathways in HEK293T cells. Meanwhile, CgIL17-5 could augment the IL6 synthesis in HuVEC cells, playing the similar roles as human IL17 in inflammatory response. Additionally, the recombinant CgIL175 (rCgIL17-5) could directly bind peptidoglycan (PGN), lipopolysaccharide (LPS), poly (I:C) and beta-1,3-glucan, with the highest affinity to PGN, and significantly inhibit the growth of Micrococcus luteus and Escherichia coli. All the results collectively suggested that CgIL17-5, as an ancient inflammatory cytokine, could not only activate signal transduction for the release of other cytokines, but also mediate the clearance of extracellular bacteria in oysters. (C) 2015 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Xin, Lusheng Zhang, Huan Zhang, Ran Li, Hui Wang, Weilin Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng |
author_facet |
Xin, Lusheng Zhang, Huan Zhang, Ran Li, Hui Wang, Weilin Wang, Lingling Wang, Hao Qiu, Limei Song, Linsheng |
author_sort |
Xin, Lusheng |
title |
CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
title_short |
CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
title_full |
CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
title_fullStr |
CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
title_full_unstemmed |
CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
title_sort |
cgil17-5, an ancient inflammatory cytokine in crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/51033 https://doi.org/10.1016/j.dci.2015.08.002 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Xin, Lusheng,Zhang, Huan,Zhang, Ran,et al. CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(2):339-348. http://ir.qdio.ac.cn/handle/337002/51033 doi:10.1016/j.dci.2015.08.002 |
op_doi |
https://doi.org/10.1016/j.dci.2015.08.002 |
container_title |
Developmental & Comparative Immunology |
container_volume |
53 |
container_issue |
2 |
container_start_page |
339 |
op_container_end_page |
348 |
_version_ |
1766393461221621760 |