A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia
calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lopho...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/50936 2023-05-15T15:58:10+02:00 A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia Wang, Xiaofei Liu, Baozhong Liu, Fengsong Huan, Pin 2015-10-01 http://ir.qdio.ac.cn/handle/337002/50936 https://doi.org/10.2108/zs150009 英语 eng ZOOLOGICAL SCIENCE Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,et al. A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia[J]. ZOOLOGICAL SCIENCE,2015,32(5):419-426. http://ir.qdio.ac.cn/handle/337002/50936 doi:10.2108/zs150009 Bivalve Calaxin Cilia Development Larva Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.2108/zs150009 2022-06-27T05:37:20Z calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lophotrochozoa species has been reported so far. This leaves open the question of whether the modulation of ciliary motility by calaxin is conserved among animals. We report a calaxin gene from the Pacific oyster Crassostrea gigas. This gene, termed cgi-calaxin, possesses three conserved EF-hand motifs as its orthologs from chordates. A phylogenetic analysis confirmed its orthology. Expression analysis revealed high expression in typical ciliated tissues such as gill and hepatopancreas. The spatiotemporal expression of the gene during early development was investigated using whole mount in situ hybridization. The results revealed that cgi-calaxin mRNA was aggregated in ciliated tissues of early larvae such as prototroch and velum. Immunofluorescence experiment further certified the ciliary localization of Cgi-Calaxin protein in D-veligers. We prepared the recombinant protein of cgi-calaxin and proved it had the capacity to bind to calcium. These results support the conserved roles of cgi-calaxin as a calcium sensor in ciliated cells and enrich the current knowledge on regulatory mechanisms of molluscan cilia. Moreover, we found the gene to be expressed in poorly ciliated tissues, such as adductor muscle, indicating possible roles in non-ciliated cells, which merits further investigation. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Zoological Science 32 5 419 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Bivalve Calaxin Cilia Development Larva |
spellingShingle |
Bivalve Calaxin Cilia Development Larva Wang, Xiaofei Liu, Baozhong Liu, Fengsong Huan, Pin A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
topic_facet |
Bivalve Calaxin Cilia Development Larva |
description |
calaxin is a newly identified calcium sensor gene that modulates the movement of flagella (and possibly cilia). It was first identified from the ascidian Ciona intestinalis, and its orthologs have been observed in a wide range of animals and choanoflagellates. However, no calaxin-ortholog in a Lophotrochozoa species has been reported so far. This leaves open the question of whether the modulation of ciliary motility by calaxin is conserved among animals. We report a calaxin gene from the Pacific oyster Crassostrea gigas. This gene, termed cgi-calaxin, possesses three conserved EF-hand motifs as its orthologs from chordates. A phylogenetic analysis confirmed its orthology. Expression analysis revealed high expression in typical ciliated tissues such as gill and hepatopancreas. The spatiotemporal expression of the gene during early development was investigated using whole mount in situ hybridization. The results revealed that cgi-calaxin mRNA was aggregated in ciliated tissues of early larvae such as prototroch and velum. Immunofluorescence experiment further certified the ciliary localization of Cgi-Calaxin protein in D-veligers. We prepared the recombinant protein of cgi-calaxin and proved it had the capacity to bind to calcium. These results support the conserved roles of cgi-calaxin as a calcium sensor in ciliated cells and enrich the current knowledge on regulatory mechanisms of molluscan cilia. Moreover, we found the gene to be expressed in poorly ciliated tissues, such as adductor muscle, indicating possible roles in non-ciliated cells, which merits further investigation. |
format |
Article in Journal/Newspaper |
author |
Wang, Xiaofei Liu, Baozhong Liu, Fengsong Huan, Pin |
author_facet |
Wang, Xiaofei Liu, Baozhong Liu, Fengsong Huan, Pin |
author_sort |
Wang, Xiaofei |
title |
A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
title_short |
A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
title_full |
A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
title_fullStr |
A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
title_full_unstemmed |
A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia |
title_sort |
calaxin gene in the pacific oyster crassostrea gigas and its potential roles in cilia |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/50936 https://doi.org/10.2108/zs150009 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
ZOOLOGICAL SCIENCE Wang, Xiaofei,Liu, Baozhong,Liu, Fengsong,et al. A calaxin Gene in the Pacific Oyster Crassostrea gigas and Its Potential Roles in Cilia[J]. ZOOLOGICAL SCIENCE,2015,32(5):419-426. http://ir.qdio.ac.cn/handle/337002/50936 doi:10.2108/zs150009 |
op_doi |
https://doi.org/10.2108/zs150009 |
container_title |
Zoological Science |
container_volume |
32 |
container_issue |
5 |
container_start_page |
419 |
_version_ |
1766393899720376320 |