ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate
Megalocytivirus, a DNA virus belonging to the Iridoviridae family, is a severe pathogen to a wide range of marine and freshwater fish. In this study, using turbot (Scophthalmus maximus) as a host model, we examined the immunoprotective property of one megalocytivirus gene, ORF75, in the form of DNA...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/49677 2023-05-15T18:15:43+02:00 ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate Zhang, Jian Li, Mo-fei 2015-08-01 http://ir.qdio.ac.cn/handle/337002/49677 https://doi.org/10.1016/j.fsi.2015.05.006 英语 eng FISH & SHELLFISH IMMUNOLOGY Zhang, Jian,Li, Mo-fei. ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):486-494. http://ir.qdio.ac.cn/handle/337002/49677 doi:10.1016/j.fsi.2015.05.006 Megalocytivirus Dna Vaccine Scophthalmus Maximus Immune Response Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.fsi.2015.05.006 2022-06-27T05:37:17Z Megalocytivirus, a DNA virus belonging to the Iridoviridae family, is a severe pathogen to a wide range of marine and freshwater fish. In this study, using turbot (Scophthalmus maximus) as a host model, we examined the immunoprotective property of one megalocytivirus gene, ORF75, in the form of DNA vaccine (named pORF75). Immunofluorescence microscopy and RT-PCR analysis showed that P444, the protein encoded by ORF75, was naturally produced in the tissues of turbot during megalocytivirus infection, and that the vaccine gene in pORF75 was expressed in fish cells transfected with pORF75 and in the tissues of turbot immunized with pORF75. Following vaccination of turbot with pORF75, a high level of survival (73%) was observed against a lethal megalocytivirus challenge. Consistently, viral replication in the vaccinated fish was significantly inhibited. Immune response analysis showed that pORF75-vaccinated fish (i) exhibited upregulated expression of the genes involved in innate and adaptive immunity, (ii) possessed specific memory immune cells that showed significant response to secondary antigen stimulation, and (iii) produced specific serum antibodies which, when co-introduced into turbot with megalocytivirus, blocked viral replication. Furthermore, whole-genome transcriptome analysis revealed that ORF75 knockdown altered the transcription of 43 viral genes. Taken together, these results indicate that ORF75 encoded a highly protective immunogen that is also a global transcription regulator of megalocytivirus. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 45 2 486 494 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Megalocytivirus Dna Vaccine Scophthalmus Maximus Immune Response |
spellingShingle |
Megalocytivirus Dna Vaccine Scophthalmus Maximus Immune Response Zhang, Jian Li, Mo-fei ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
topic_facet |
Megalocytivirus Dna Vaccine Scophthalmus Maximus Immune Response |
description |
Megalocytivirus, a DNA virus belonging to the Iridoviridae family, is a severe pathogen to a wide range of marine and freshwater fish. In this study, using turbot (Scophthalmus maximus) as a host model, we examined the immunoprotective property of one megalocytivirus gene, ORF75, in the form of DNA vaccine (named pORF75). Immunofluorescence microscopy and RT-PCR analysis showed that P444, the protein encoded by ORF75, was naturally produced in the tissues of turbot during megalocytivirus infection, and that the vaccine gene in pORF75 was expressed in fish cells transfected with pORF75 and in the tissues of turbot immunized with pORF75. Following vaccination of turbot with pORF75, a high level of survival (73%) was observed against a lethal megalocytivirus challenge. Consistently, viral replication in the vaccinated fish was significantly inhibited. Immune response analysis showed that pORF75-vaccinated fish (i) exhibited upregulated expression of the genes involved in innate and adaptive immunity, (ii) possessed specific memory immune cells that showed significant response to secondary antigen stimulation, and (iii) produced specific serum antibodies which, when co-introduced into turbot with megalocytivirus, blocked viral replication. Furthermore, whole-genome transcriptome analysis revealed that ORF75 knockdown altered the transcription of 43 viral genes. Taken together, these results indicate that ORF75 encoded a highly protective immunogen that is also a global transcription regulator of megalocytivirus. (C) 2015 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Zhang, Jian Li, Mo-fei |
author_facet |
Zhang, Jian Li, Mo-fei |
author_sort |
Zhang, Jian |
title |
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
title_short |
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
title_full |
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
title_fullStr |
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
title_full_unstemmed |
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate |
title_sort |
orf75 of megalocytivirus rbiv-c1: a global transcription regulator and an effective vaccine candidate |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/49677 https://doi.org/10.1016/j.fsi.2015.05.006 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Zhang, Jian,Li, Mo-fei. ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):486-494. http://ir.qdio.ac.cn/handle/337002/49677 doi:10.1016/j.fsi.2015.05.006 |
op_doi |
https://doi.org/10.1016/j.fsi.2015.05.006 |
container_title |
Fish & Shellfish Immunology |
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45 |
container_issue |
2 |
container_start_page |
486 |
op_container_end_page |
494 |
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1766188924555755520 |