Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present w...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/49204 2023-05-15T18:15:51+02:00 Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis Liu, Li Chi, Heng Sun, Li 2015-06-29 http://ir.qdio.ac.cn/handle/337002/49204 https://doi.org/10.3354/dao02874 英语 eng DISEASES OF AQUATIC ORGANISMS Liu, Li,Chi, Heng,Sun, Li. Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis[J]. DISEASES OF AQUATIC ORGANISMS,2015,115(1):67-80. http://ir.qdio.ac.cn/handle/337002/49204 doi:10.3354/dao02874 Pseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.3354/dao02874 2022-06-27T05:37:17Z Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories. Four of these proteins, viz. TssP (a type VI secretion protein), PspA (a serine protease), OprF (an outer membrane porin), and ClpP (the proteolytic subunit of an ATP-dependent Clp protease), were assessed for virulence participation in a model of turbot Scophthalmus maximus. The results showed that the oprF and clpP knockouts exhibited significantly reduced capacities in (1) resistance against the bactericidal effect of host serum, (2) dissemination into and colonization of host tissues, and (3) inducing host mortality. In contrast, mutation of tssP and pspA had no apparent effect on the pathogenicity of TSS. Purified recombinant OprF, when used as a subunit vaccine, induced production of specific serum antibodies in immunized fish and elicited significant protection against lethal TSS challenge. Antibody blocking of the OprF in TSS significantly impaired the ability of the bacteria to invade host tissues. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, Fur regulates the expression of diverse proteins, some of which are required for optimal infection. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Diseases of Aquatic Organisms 115 1 67 80 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine |
spellingShingle |
Pseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine Liu, Li Chi, Heng Sun, Li Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
topic_facet |
Pseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine |
description |
Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories. Four of these proteins, viz. TssP (a type VI secretion protein), PspA (a serine protease), OprF (an outer membrane porin), and ClpP (the proteolytic subunit of an ATP-dependent Clp protease), were assessed for virulence participation in a model of turbot Scophthalmus maximus. The results showed that the oprF and clpP knockouts exhibited significantly reduced capacities in (1) resistance against the bactericidal effect of host serum, (2) dissemination into and colonization of host tissues, and (3) inducing host mortality. In contrast, mutation of tssP and pspA had no apparent effect on the pathogenicity of TSS. Purified recombinant OprF, when used as a subunit vaccine, induced production of specific serum antibodies in immunized fish and elicited significant protection against lethal TSS challenge. Antibody blocking of the OprF in TSS significantly impaired the ability of the bacteria to invade host tissues. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, Fur regulates the expression of diverse proteins, some of which are required for optimal infection. |
format |
Article in Journal/Newspaper |
author |
Liu, Li Chi, Heng Sun, Li |
author_facet |
Liu, Li Chi, Heng Sun, Li |
author_sort |
Liu, Li |
title |
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
title_short |
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
title_full |
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
title_fullStr |
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
title_full_unstemmed |
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis |
title_sort |
pseudomonas fluorescens: identification of fur-regulated proteins and evaluation of their contribution to pathogenesis |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/49204 https://doi.org/10.3354/dao02874 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
DISEASES OF AQUATIC ORGANISMS Liu, Li,Chi, Heng,Sun, Li. Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis[J]. DISEASES OF AQUATIC ORGANISMS,2015,115(1):67-80. http://ir.qdio.ac.cn/handle/337002/49204 doi:10.3354/dao02874 |
op_doi |
https://doi.org/10.3354/dao02874 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
115 |
container_issue |
1 |
container_start_page |
67 |
op_container_end_page |
80 |
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1766189082063405056 |