Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense
The inhibitor of apoptosis (IAP) proteins maintain a balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses. In this study, phylogenetic analysis revealed lineage-specific expansion and tandem duplication of IAPs in the Pacific oyster Crass...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/49050 https://doi.org/10.1016/j.dci.2015.02.011 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/49050 2023-05-15T15:57:34+02:00 Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense Qu, Tao Zhang, Linlin Wang, Wei Huang, Baoyu Li, Yingxiang Zhu, Qihui Li, Li Zhang, Guofan 2015-07-01 http://ir.qdio.ac.cn/handle/337002/49050 https://doi.org/10.1016/j.dci.2015.02.011 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Qu, Tao,Zhang, Linlin,Wang, Wei,et al. Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):74-78. http://ir.qdio.ac.cn/handle/337002/49050 doi:10.1016/j.dci.2015.02.011 Crassostrea Gigas Iap Evolution Intrinsic Apoptosis Caspase Immune Response Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.dci.2015.02.011 2022-06-27T05:37:17Z The inhibitor of apoptosis (IAP) proteins maintain a balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses. In this study, phylogenetic analysis revealed lineage-specific expansion and tandem duplication of IAPs in the Pacific oyster Crassostrea gigas. We then investigated a representative oyster-specific XIAP-like gene (CgIAP2) to understand how it regulates initiator caspase. Cloning of full-length CgIAP2 from oyster cDNA uncovered a deduced protein containing two BIR domains and a RING domain. Homolog comparison demonstrated that CgIAP2 clustered into the invertebrate branch. We found that CgIAP2 was likely involved in apoptosis inhibition and immune defense, based on high mRNA expression in the gills and labial palps, as well as increased mRNA expression after bacterial challenge. A yeast two-hybrid assay revealed that the BIR2 domain was necessary and sufficient to mediate interaction between CgIAP2 and Cgcaspase-2, providing direct evidence that CgIAP2 participates in apoptosis inhibition. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 51 1 74 78 |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Iap Evolution Intrinsic Apoptosis Caspase Immune Response |
spellingShingle |
Crassostrea Gigas Iap Evolution Intrinsic Apoptosis Caspase Immune Response Qu, Tao Zhang, Linlin Wang, Wei Huang, Baoyu Li, Yingxiang Zhu, Qihui Li, Li Zhang, Guofan Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
topic_facet |
Crassostrea Gigas Iap Evolution Intrinsic Apoptosis Caspase Immune Response |
description |
The inhibitor of apoptosis (IAP) proteins maintain a balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses. In this study, phylogenetic analysis revealed lineage-specific expansion and tandem duplication of IAPs in the Pacific oyster Crassostrea gigas. We then investigated a representative oyster-specific XIAP-like gene (CgIAP2) to understand how it regulates initiator caspase. Cloning of full-length CgIAP2 from oyster cDNA uncovered a deduced protein containing two BIR domains and a RING domain. Homolog comparison demonstrated that CgIAP2 clustered into the invertebrate branch. We found that CgIAP2 was likely involved in apoptosis inhibition and immune defense, based on high mRNA expression in the gills and labial palps, as well as increased mRNA expression after bacterial challenge. A yeast two-hybrid assay revealed that the BIR2 domain was necessary and sufficient to mediate interaction between CgIAP2 and Cgcaspase-2, providing direct evidence that CgIAP2 participates in apoptosis inhibition. (C) 2015 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Qu, Tao Zhang, Linlin Wang, Wei Huang, Baoyu Li, Yingxiang Zhu, Qihui Li, Li Zhang, Guofan |
author_facet |
Qu, Tao Zhang, Linlin Wang, Wei Huang, Baoyu Li, Yingxiang Zhu, Qihui Li, Li Zhang, Guofan |
author_sort |
Qu, Tao |
title |
Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
title_short |
Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
title_full |
Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
title_fullStr |
Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
title_full_unstemmed |
Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense |
title_sort |
characterization of an inhibitor of apoptosis protein in crassostrea gigas clarifies its role in apoptosis and immune defense |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/49050 https://doi.org/10.1016/j.dci.2015.02.011 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Qu, Tao,Zhang, Linlin,Wang, Wei,et al. Characterization of an inhibitor of apoptosis protein in Crassostrea gigas clarifies its role in apoptosis and immune defense[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):74-78. http://ir.qdio.ac.cn/handle/337002/49050 doi:10.1016/j.dci.2015.02.011 |
op_doi |
https://doi.org/10.1016/j.dci.2015.02.011 |
container_title |
Developmental & Comparative Immunology |
container_volume |
51 |
container_issue |
1 |
container_start_page |
74 |
op_container_end_page |
78 |
_version_ |
1766393243227914240 |