AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg
Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/3564 2024-09-15T18:03:05+00:00 AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg Li, L Guo, XM 2004 http://ir.qdio.ac.cn/handle/337002/3565 https://doi.org/10.1007/s10126-003-0001-0 英语 eng MARINE BIOTECHNOLOGY Li, L; Guo, XM.AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg,MARINE BIOTECHNOLOGY,2004,6(1):26-36 http://ir.qdio.ac.cn/handle/337002/3565 doi:10.1007/s10126-003-0001-0 Linkage Mapping Genetic Map Aflp Genome Oyster Crassostrea Gigas Biotechnology & Applied Microbiology Marine & Freshwater Biology Article 期刊论文 2004 ftchinacasciocas https://doi.org/10.1007/s10126-003-0001-0 2024-08-09T03:18:11Z Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The estimated genome length of the Pacific oyster was 1258 cM for the female and 933 cM for the male, and the observed coverage was 82.0% for the female map and 81.3% for the male map. Most distorted markers were deficient for homozygotes and closely linked to each other on the genetic map, suggesting the presence of major recessive deleterious genes in the Pacific oyster. Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Marine Biotechnology 6 1 26 36 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Linkage Mapping Genetic Map Aflp Genome Oyster Crassostrea Gigas Biotechnology & Applied Microbiology Marine & Freshwater Biology |
spellingShingle |
Linkage Mapping Genetic Map Aflp Genome Oyster Crassostrea Gigas Biotechnology & Applied Microbiology Marine & Freshwater Biology Li, L Guo, XM AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
topic_facet |
Linkage Mapping Genetic Map Aflp Genome Oyster Crassostrea Gigas Biotechnology & Applied Microbiology Marine & Freshwater Biology |
description |
Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The estimated genome length of the Pacific oyster was 1258 cM for the female and 933 cM for the male, and the observed coverage was 82.0% for the female map and 81.3% for the male map. Most distorted markers were deficient for homozygotes and closely linked to each other on the genetic map, suggesting the presence of major recessive deleterious genes in the Pacific oyster. Amplified fragment length polymorphisms (AFLPs) were used for genome mapping in the Pacific Oyster Crassostrea gigas Thunberg. Seventeen selected primer combinations produced 1106 peaks, of which 384 (34.7%) were polymorphic in a backcross family. Among the polymorphic markers, 349 were segregating through either the female or the male parent. Chi-square analysis indicated that 255 (73.1%) of the markers segregated in a Mendelian ratio, and 94 (26.9%) showed significant (P < 0.05) segregation distortion. Separate genetic linkage maps were constructed for the female and male parents. The female framework map consisted of 119 markers in 11 linkage groups, spanning 1030.7 cM, with an average interval of 9.5 cM per marker. The male map contained 96 markers in 10 linkage groups, covering 758.4 cM, with 8.8 cM per marker. The ... |
format |
Article in Journal/Newspaper |
author |
Li, L Guo, XM |
author_facet |
Li, L Guo, XM |
author_sort |
Li, L |
title |
AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
title_short |
AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
title_full |
AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
title_fullStr |
AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
title_full_unstemmed |
AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg |
title_sort |
aflp-based genetic linkage maps of the pacific oyster crassostrea gigas thunberg |
publishDate |
2004 |
url |
http://ir.qdio.ac.cn/handle/337002/3565 https://doi.org/10.1007/s10126-003-0001-0 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE BIOTECHNOLOGY Li, L; Guo, XM.AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg,MARINE BIOTECHNOLOGY,2004,6(1):26-36 http://ir.qdio.ac.cn/handle/337002/3565 doi:10.1007/s10126-003-0001-0 |
op_doi |
https://doi.org/10.1007/s10126-003-0001-0 |
container_title |
Marine Biotechnology |
container_volume |
6 |
container_issue |
1 |
container_start_page |
26 |
op_container_end_page |
36 |
_version_ |
1810440611638018048 |