A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system

C-type lectins (CTLs), serving as pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that participate in nonself-recognition and pathogen elimination. In the present study, a single carbohydrate-recognition domain (CRD) CTL was identified from...

Full description

Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Li, Hui, Zhang, Huan, Jiang, Shuai, Wang, Weilin, Xin, Lusheng, Wang, Hao, Wang, Lingling, Song, Linsheng
Format: Article in Journal/Newspaper
Language:English
Published: 2015
Subjects:
Crassostrea Gigas
C-type Lectin
Innate Immunity
Prr
Opsonin
Growth Suppression Activity
Complement System
Crassostrea gigas
Online Access:http://ir.qdio.ac.cn/handle/337002/26971
https://doi.org/10.1016/j.fsi.2015.03.011
Description
Summary:C-type lectins (CTLs), serving as pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that participate in nonself-recognition and pathogen elimination. In the present study, a single carbohydrate-recognition domain (CRD) CTL was identified from oyster Crassostrea gigas (designated as CgCLec-2). There was only one CRD within the deduced amino acid sequence of CgCLec-2 consisting of 129 amino acid residues. A conserved EPN (Glu(246)-Pro(247)-Asn(248)) motif was found in Ca2+-binding site 2 of CgCLec-2. The CgCLec-2 mRNA could be detected in all the examined tissues at different expression levels in oysters. The mRNA expression of CgCLec-2 in hemocytes was up-regulated significantly at 6 h post Vibrio splendidus challenge. The recombinant CgCLec-2 (rCgCLec-2) could bind various Pathogen-Associated Molecular Patterns (PAMPs), including lipopolysaccharide, mannan and peptidoglycan, and displayed strong binding abilities to Vibrio anguillarum, V. splendidus and Yarrowiali polytica and week binding ability to Staphylococcus aureus. It could also enhance the phagocytic activity of oyster hemocytes to V. splendidus and exhibited growth suppression activity against gram-positive bacteria S. aureus but no effect on gram-negative bacteria V. splendidus. Furthermore, the interaction between rCgCLec-2 and rCgMASPL-1 was confirmed by GST Pull down. The results suggested that CgCLec-2 served as not only a PRR in immune recognition but also a regulatory factor in pathogen elimination, and played a potential role in the activation of complement system. (c) 2015 Elsevier Ltd. All rights reserved.

Similar Items