The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L)
Enzyme activities and gene expression of a number of innate immune parameters in the serum, mucus and skin of Atlantic salmon (Salmo salar) were investigated after challenge with a pathogenic strain of Aeromonas salmonicida (A. salmonicida). Fish were injected in the dorsal muscle with either 100 mu...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/26951 https://doi.org/10.1016/j.fsi.2015.02.029 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/26951 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/26951 2023-05-15T15:30:25+02:00 The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) Du, Yishuai Yi, Mengmeng Xiao, Peng Meng, Lingjie Li, Xian Sun, Guoxiang Liu, Ying 2015-05-01 http://ir.qdio.ac.cn/handle/337002/26951 https://doi.org/10.1016/j.fsi.2015.02.029 英语 eng FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/26951 doi:10.1016/j.fsi.2015.02.029 Atlantic Salmon Aeromonas Salmonicida Innate Immunity Immune Response Early-warning Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.fsi.2015.02.029 2022-06-27T05:36:58Z Enzyme activities and gene expression of a number of innate immune parameters in the serum, mucus and skin of Atlantic salmon (Salmo salar) were investigated after challenge with a pathogenic strain of Aeromonas salmonicida (A. salmonicida). Fish were injected in the dorsal muscle with either 100 mu l bacterium solution, about 3.05 x 10(7) CPU/ml A. scdmonicida, or 100 mu l 0.9% NaCl (as control group) and tissue samples were collected at days 0, 2, 4 and 6 post-injection. Lysozyme (LSZ) and alkaline phosphatase (AKP) activities in serum, mucus and skin, and LSZ and AKP mRNA expression in skin of the challenged fish were higher than those of the control at most of the experimental time, with significant differences at several time points (P < 0.05), indicating the involvement of LSZ and AKP in the innate immunity of Atlantic salmon to A. salmonicida. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in mucus and skin, along with the SOD, POD and CAT mRNA expression in skin significantly decreased at day 4 and 6, indicating the decreased antioxidant capacity of the challenged fish. Glutamate pyruvate transaminase (GPT) and glutamic oxalacetic transaminase (GOT) activities in serum, mucus and skin of the challenged group were all higher than those of the control after the injection, and at several time points significant differences were found between the two groups, suggesting organs of fish were impaired after the pathogen infection. The changes of the GPT and GOT activities could be used as potential biomarkers for the impairment of physiological functions caused by the pathogen infection. Identified biomarkers of the immune responses will contribute to the early-warning system of the disease. So this study will not only provide a theoretical basis for vaccine development, but also provide basic data for the establishment of early warning systems for diseases caused by A. salmonicida in Atlantic salmon rearing. (C) 2015 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 44 1 307 315 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Atlantic Salmon Aeromonas Salmonicida Innate Immunity Immune Response Early-warning |
spellingShingle |
Atlantic Salmon Aeromonas Salmonicida Innate Immunity Immune Response Early-warning Du, Yishuai Yi, Mengmeng Xiao, Peng Meng, Lingjie Li, Xian Sun, Guoxiang Liu, Ying The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
topic_facet |
Atlantic Salmon Aeromonas Salmonicida Innate Immunity Immune Response Early-warning |
description |
Enzyme activities and gene expression of a number of innate immune parameters in the serum, mucus and skin of Atlantic salmon (Salmo salar) were investigated after challenge with a pathogenic strain of Aeromonas salmonicida (A. salmonicida). Fish were injected in the dorsal muscle with either 100 mu l bacterium solution, about 3.05 x 10(7) CPU/ml A. scdmonicida, or 100 mu l 0.9% NaCl (as control group) and tissue samples were collected at days 0, 2, 4 and 6 post-injection. Lysozyme (LSZ) and alkaline phosphatase (AKP) activities in serum, mucus and skin, and LSZ and AKP mRNA expression in skin of the challenged fish were higher than those of the control at most of the experimental time, with significant differences at several time points (P < 0.05), indicating the involvement of LSZ and AKP in the innate immunity of Atlantic salmon to A. salmonicida. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in mucus and skin, along with the SOD, POD and CAT mRNA expression in skin significantly decreased at day 4 and 6, indicating the decreased antioxidant capacity of the challenged fish. Glutamate pyruvate transaminase (GPT) and glutamic oxalacetic transaminase (GOT) activities in serum, mucus and skin of the challenged group were all higher than those of the control after the injection, and at several time points significant differences were found between the two groups, suggesting organs of fish were impaired after the pathogen infection. The changes of the GPT and GOT activities could be used as potential biomarkers for the impairment of physiological functions caused by the pathogen infection. Identified biomarkers of the immune responses will contribute to the early-warning system of the disease. So this study will not only provide a theoretical basis for vaccine development, but also provide basic data for the establishment of early warning systems for diseases caused by A. salmonicida in Atlantic salmon rearing. (C) 2015 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Du, Yishuai Yi, Mengmeng Xiao, Peng Meng, Lingjie Li, Xian Sun, Guoxiang Liu, Ying |
author_facet |
Du, Yishuai Yi, Mengmeng Xiao, Peng Meng, Lingjie Li, Xian Sun, Guoxiang Liu, Ying |
author_sort |
Du, Yishuai |
title |
The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
title_short |
The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
title_full |
The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
title_fullStr |
The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
title_full_unstemmed |
The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L) |
title_sort |
impact of aeromonas salmonicida infection on innate immune parameters of atlantic salmon (salmo salar l) |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/26951 https://doi.org/10.1016/j.fsi.2015.02.029 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/26951 doi:10.1016/j.fsi.2015.02.029 |
op_doi |
https://doi.org/10.1016/j.fsi.2015.02.029 |
container_title |
Fish & Shellfish Immunology |
container_volume |
44 |
container_issue |
1 |
container_start_page |
307 |
op_container_end_page |
315 |
_version_ |
1766360866317402112 |