Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection
For pathogenic bacteria, the ability to acquire iron is vital to survival in the host. In consequence, many genes involved in iron acquisition are associated with bacterial virulence. Pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. However, the global regulatory function...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/25461 2023-05-15T18:15:51+02:00 Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection Sun, Yuan-yuan Sun, Li 2015-04-17 http://ir.qdio.ac.cn/handle/337002/25461 https://doi.org/10.1016/j.vetmic.2015.01.020 英语 eng VETERINARY MICROBIOLOGY http://ir.qdio.ac.cn/handle/337002/25461 doi:10.1016/j.vetmic.2015.01.020 Pseudomonas Fluorescens Iron Proteomic Virulence Immunoprotection Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.vetmic.2015.01.020 2022-06-27T05:36:55Z For pathogenic bacteria, the ability to acquire iron is vital to survival in the host. In consequence, many genes involved in iron acquisition are associated with bacterial virulence. Pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. However, the global regulatory function of iron in pathogenic P. fluorescens is essentially unknown. In this study, in order to identify proteins affected by iron condition at the expression level, we performed proteomic analysis to compare the global protein profiles of P. fluorescens strain TSS, a fish pathogen, cultured under iron-replete and iron-deplete conditions. Twenty-two differentially expressed proteins were identified, most of which were confirmed to be regulated by iron at the mRNA level. To investigate their potential involvement in virulence, the genes encoding four of the 22 proteins, i.e. HemO (heme oxygenase), PspB (serine protease), Sod (superoxide dismutase), and TfeR (TonB-dependent outermembrane ferric enterobactin receptor), were knocked out, and the pathogenicity of the mutants was examined in a model of turbot (Scophthalmus maximus). The results showed that compared to the wild type, the hemO, pspB, and tfeR knockouts were significantly impaired in the ability to survive in host serum, to invade host tissues, and to cause host mortality. Immunization of turbot with recombinant TfeR (rTfeR) and PspB induced production of specific serum antibodies and significant protections against lethal TSS challenge. Further analysis showed that rTfeR antibodies recognized and bound to TSS, and that treatment of TSS with rTfeR antibodies significantly impaired the infectivity of TSS to fish cells. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, iron affects the expression of a large number of proteins including those that are involved in host infection. (C) 2015 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Veterinary Microbiology 176 3-4 309 320 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pseudomonas Fluorescens Iron Proteomic Virulence Immunoprotection |
spellingShingle |
Pseudomonas Fluorescens Iron Proteomic Virulence Immunoprotection Sun, Yuan-yuan Sun, Li Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
topic_facet |
Pseudomonas Fluorescens Iron Proteomic Virulence Immunoprotection |
description |
For pathogenic bacteria, the ability to acquire iron is vital to survival in the host. In consequence, many genes involved in iron acquisition are associated with bacterial virulence. Pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. However, the global regulatory function of iron in pathogenic P. fluorescens is essentially unknown. In this study, in order to identify proteins affected by iron condition at the expression level, we performed proteomic analysis to compare the global protein profiles of P. fluorescens strain TSS, a fish pathogen, cultured under iron-replete and iron-deplete conditions. Twenty-two differentially expressed proteins were identified, most of which were confirmed to be regulated by iron at the mRNA level. To investigate their potential involvement in virulence, the genes encoding four of the 22 proteins, i.e. HemO (heme oxygenase), PspB (serine protease), Sod (superoxide dismutase), and TfeR (TonB-dependent outermembrane ferric enterobactin receptor), were knocked out, and the pathogenicity of the mutants was examined in a model of turbot (Scophthalmus maximus). The results showed that compared to the wild type, the hemO, pspB, and tfeR knockouts were significantly impaired in the ability to survive in host serum, to invade host tissues, and to cause host mortality. Immunization of turbot with recombinant TfeR (rTfeR) and PspB induced production of specific serum antibodies and significant protections against lethal TSS challenge. Further analysis showed that rTfeR antibodies recognized and bound to TSS, and that treatment of TSS with rTfeR antibodies significantly impaired the infectivity of TSS to fish cells. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, iron affects the expression of a large number of proteins including those that are involved in host infection. (C) 2015 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Sun, Yuan-yuan Sun, Li |
author_facet |
Sun, Yuan-yuan Sun, Li |
author_sort |
Sun, Yuan-yuan |
title |
Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
title_short |
Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
title_full |
Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
title_fullStr |
Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
title_full_unstemmed |
Pseudomonas fluorescens: Iron-responsive proteins and their involvement in host infection |
title_sort |
pseudomonas fluorescens: iron-responsive proteins and their involvement in host infection |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/25461 https://doi.org/10.1016/j.vetmic.2015.01.020 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
VETERINARY MICROBIOLOGY http://ir.qdio.ac.cn/handle/337002/25461 doi:10.1016/j.vetmic.2015.01.020 |
op_doi |
https://doi.org/10.1016/j.vetmic.2015.01.020 |
container_title |
Veterinary Microbiology |
container_volume |
176 |
container_issue |
3-4 |
container_start_page |
309 |
op_container_end_page |
320 |
_version_ |
1766189096054554624 |