Expression and enzymatic characterization of a cold-adapted beta-agarase from Antarctic bacterium Pseudoalteromonas sp NJ21

• Published in: Chinese Journal of Oceanology and Limnology
• Main Authors: Li Jiang, Sha Yujie
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2015
• Subjects: Antarctic Bacterium Pseudoalteromonas; Agarase; Expression; Enzymatic Characterization; Antarctic; The Antarctic; Antarc*
• Online Access: http://ir.qdio.ac.cn/handle/337002/25403; https://doi.org/10.1007/s00343-015-4072-3

An agar-degrading bacterium, designated as Pseudoalteromonas sp. NJ21, was isolated from an Antarctic sediment sample. The agarase gene aga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase (rAga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30-40A degrees C and 8.0, respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10A degrees C, which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an beta-agarase, producing neoagarobiose (NA2) as the final main product. Furthermore, this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.