The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas
Opioid receptors (OR) are a group of G protein-coupled receptors with opioids as ligands, which play an important role in triggering the second messengers to modulate immune response in vertebrate immunocytes. In the present study, the full length cDNA of a homologue of delta-opioid receptor (DOR) f...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/25370 2023-05-15T15:57:46+02:00 The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas Liu, Zhaoqun Zhou, Zhi Wang, Lingling Jiang, Shuai Wang, Weilin Zhang, Ran Song, Linsheng 2015-04-01 http://ir.qdio.ac.cn/handle/337002/25370 https://doi.org/10.1016/j.dci.2014.11.017 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/25370 doi:10.1016/j.dci.2014.11.017 Crassostrea Gigas Delta-opioid Receptor [Met(5)]-enkephalin Immunomodulation Haemocyte Article 期刊论文 2015 ftchinacasciocas https://doi.org/10.1016/j.dci.2014.11.017 2022-06-27T05:36:52Z Opioid receptors (OR) are a group of G protein-coupled receptors with opioids as ligands, which play an important role in triggering the second messengers to modulate immune response in vertebrate immunocytes. In the present study, the full length cDNA of a homologue of delta-opioid receptor (DOR) for [Met(5)]-enkaphalin was cloned from oyster Crassostrea gigas (designated as CgDOR), which was 1104 bp encoding a peptide of 367 amino acids containing a conserved 7tm_1 domain. After the stimulation of [Met(5)]-enkephalin, the concentration of second messengers Ca2+ and cAMP in the HEK293T cells decreased significantly (p < 0.05) with the expression of CgDOR. However, this trend was reverted with the addition of DOR antagonist BNTX. The CgDOR transcripts were ubiquitously detected in the tested tissues including haemocytes, gonad, mantle, kidney, gill, adductor muscle and hepatopancreas, with the highest expression level in the hepatopancreas. After LPS stimulation, the expression level of CgDOR mRNA began to increase (4.05-fold, p < 0.05) at 6 h, and reached the highest level (5.00-fold, p < 0.05) at 12 h. Haemocyte phagocytic and antibacterial activities increased significantly after [Met(5)]-enkephalin stimulation, whereas the increase was repressed with the addition of DOR antagonist BNTX. These results collectively suggested that CgDOR for [Met(5)]-enkephalin could modulate the haemocyte phagocytic and antibacterial functions through the second messengers Ca2+ and cAMP, which might be requisite for pathogen elimination and homeostasis maintenance in oyster. (C) 2014 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 49 2 217 224 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Delta-opioid Receptor [Met(5)]-enkephalin Immunomodulation Haemocyte |
spellingShingle |
Crassostrea Gigas Delta-opioid Receptor [Met(5)]-enkephalin Immunomodulation Haemocyte Liu, Zhaoqun Zhou, Zhi Wang, Lingling Jiang, Shuai Wang, Weilin Zhang, Ran Song, Linsheng The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
topic_facet |
Crassostrea Gigas Delta-opioid Receptor [Met(5)]-enkephalin Immunomodulation Haemocyte |
description |
Opioid receptors (OR) are a group of G protein-coupled receptors with opioids as ligands, which play an important role in triggering the second messengers to modulate immune response in vertebrate immunocytes. In the present study, the full length cDNA of a homologue of delta-opioid receptor (DOR) for [Met(5)]-enkaphalin was cloned from oyster Crassostrea gigas (designated as CgDOR), which was 1104 bp encoding a peptide of 367 amino acids containing a conserved 7tm_1 domain. After the stimulation of [Met(5)]-enkephalin, the concentration of second messengers Ca2+ and cAMP in the HEK293T cells decreased significantly (p < 0.05) with the expression of CgDOR. However, this trend was reverted with the addition of DOR antagonist BNTX. The CgDOR transcripts were ubiquitously detected in the tested tissues including haemocytes, gonad, mantle, kidney, gill, adductor muscle and hepatopancreas, with the highest expression level in the hepatopancreas. After LPS stimulation, the expression level of CgDOR mRNA began to increase (4.05-fold, p < 0.05) at 6 h, and reached the highest level (5.00-fold, p < 0.05) at 12 h. Haemocyte phagocytic and antibacterial activities increased significantly after [Met(5)]-enkephalin stimulation, whereas the increase was repressed with the addition of DOR antagonist BNTX. These results collectively suggested that CgDOR for [Met(5)]-enkephalin could modulate the haemocyte phagocytic and antibacterial functions through the second messengers Ca2+ and cAMP, which might be requisite for pathogen elimination and homeostasis maintenance in oyster. (C) 2014 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Liu, Zhaoqun Zhou, Zhi Wang, Lingling Jiang, Shuai Wang, Weilin Zhang, Ran Song, Linsheng |
author_facet |
Liu, Zhaoqun Zhou, Zhi Wang, Lingling Jiang, Shuai Wang, Weilin Zhang, Ran Song, Linsheng |
author_sort |
Liu, Zhaoqun |
title |
The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
title_short |
The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
title_full |
The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
title_fullStr |
The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
title_full_unstemmed |
The immunomodulation mediated by a delta-opioid receptor for [Met(5)]-enkephalin in oyster Crassostrea gigas |
title_sort |
immunomodulation mediated by a delta-opioid receptor for [met(5)]-enkephalin in oyster crassostrea gigas |
publishDate |
2015 |
url |
http://ir.qdio.ac.cn/handle/337002/25370 https://doi.org/10.1016/j.dci.2014.11.017 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/25370 doi:10.1016/j.dci.2014.11.017 |
op_doi |
https://doi.org/10.1016/j.dci.2014.11.017 |
container_title |
Developmental & Comparative Immunology |
container_volume |
49 |
container_issue |
2 |
container_start_page |
217 |
op_container_end_page |
224 |
_version_ |
1766393460874543104 |