A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis

• Published in: Chinese Journal of Oceanology and Limnology
• Main Authors: Wang Jiafeng, Xu Fei, Li Li, Zhang Guofan, Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2014
• Subjects: Identification; Cytochrome Oxidase Subunit i (Coi); Crassostrea; Oyster; High-resolution Melting (Hrm); Marine & Freshwater Biology; Oceanography; Pacific; Pacific oyster
• Online Access: http://ir.qdio.ac.cn/handle/337002/24194; https://doi.org/10.1007/s00343-014-3124-4

The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves. However, because DNA can exhibit marked differences among morphologically similar species, DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis, Jinjiang oyster C. ariakensis, Portuguese oyster C. angulata, Kumamoto oyster C. sikamea, and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform, we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient, reliable, and easy to visualize. Using a single pair of primers (Oyster-COI-1), we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.